Thymosin β4 is not required for embryonic viability or vascular development.
Thymosin β4 is not required for embryonic viability or vascular development.
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DOI：
10.1161/circresaha.111.300197
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发表时间：
2013-02-01
影响因子：
20.1
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Banerjee I;Moore Morris T;Evans SM;Chen J
Rossdeutsch等人。描述胸腺素β4(Tβ4)在血管发育中的需求。通过分析两个品系的小鼠，观察到壁细胞迁移、分化、部分胚胎死亡和出血，其中一个品系的Tβ4为零，另一个品系的Tβ4被内皮细胞特异性表达的Tβ4shRNA击倒。这些数据与我们发表的全球和心脏特异性Tβ4基因敲除株系形成了直接对比。因此，需要阐明Tβ4的作用，以了解其在心血管发育中的重要性。探讨和阐明T-β-4在血管平滑肌细胞发育和血管稳定性中的作用。对Tβ4整体基因敲除的检查没有显示胚胎出血、壁细胞发育或致死性改变。内皮细胞特异性基因敲除也没有表现出任何胚胎致死性，并且可以存活到成年。对我们的Tβ4全局以及心脏和血管内皮细胞特异性基因敲除模型的分析表明，Tβ4对于胚胎存活和血管发育是必不可少的。
Rossdeutsch et al. describe a requirement for Thymosin β4 (Tβ4) in vascular development. Impaired mural cell migration, differentiation, partial embryonic lethality, and hemorrhaging were observed following analysis of two lines of mice, one of which was germline null for Tβ4, and another in which Tβ4 was knocked down by endothelial specific expression of Tβ4 shRNA. These data are in direct contrast to our published global and cardiac specific Tβ4 knockout lines. Thus the role of Tβ4 needs to be clarified to understand its importance in cardiovascular development. To investigate and clarify the role of Tβ4 in vascular smooth muscle cell development and vessel stability. Examination of Tβ4 global knockouts did not demonstrate embryonic hemorrhaging, altered mural cell development or lethality. Endothelial specific knockouts also did not exhibit any embryonic lethality and were viable to adulthood. Analysis of our Tβ4 global and cardiac- and endothelial-specific knockout models demonstrated that Tβ4 is dispensable for embryonic viability and vascular development.