Structural characterization of plant defensin protein superfamily
Structural characterization of plant defensin protein superfamily
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DOI：
10.1007/s11033-011-1235-y
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发表时间：
2012-04-01
影响因子：
2.8
通讯作者：
Kotchoni, Simeon O.
中科院分区：
文献类型：
--
作者：
Gachomo, Emma W.;Jimenez-Lopez, Jose C.;Kotchoni, Simeon O.
植物防御素是一个主要的天然免疫蛋白超家族，对人类感染性疾病具有很强的抑制作用，具有抗真菌/抗菌活性、蛋白酶和昆虫淀粉酶抑制活性。它们通常由其保守的半胱氨酸支架和连接到支架上的α-螺旋和三链反平行的β-折叠来定义。随着更多植物的基因组被完全测序，关于新测序的防御素蛋白的重要信息也被揭示出来。在本论文中，我们鉴定了植物防御素蛋白家族的成员，并利用基于蛋白质模型的结构重组来揭示植物防御素在植物中介导防御反应和其他有趣的生物学活动的特定三维隐藏特征。我们的数据显示，尽管植物防御素和昆虫防御素的氨基酸序列相似性很低，但它们在结构上与昆虫防御素相似。讨论了植物防御素和其他物种防御素之间的分子和结构关系。
Plant defensins represent a major innate immune protein superfamily with strong inhibitory effects on infectious diseases of humans, antifungal/antibacterial activities, proteinase and insect amylase inhibitory activities. They are generally defined by their conserved cysteine scaffold with alpha-helix and triple strand anti parallel beta-sheet connected to the scaffold. With the genome of more plant species being fully sequenced, significant information about newly sequenced defensin proteins has been revealed. In this paper, we identify members of defensin protein families across plant species and use protein-modeling-based structural reconstitution to reveal specific three dimensional hidden features of plant defensins mediating defense responses and other interesting biological activities in plants. Our data revealed that plant defensins are structurally similar to their insect counterparts despite the low amino acid sequence similarity between these two organisms. The molecular and structural relationship among plant defensins and defensins from other species is discussed.