Study on saccharification techniques of seaweed wastes for the transformation of ethanol
Study on saccharification techniques of seaweed wastes for the transformation of ethanol
复制标题
海藻废弃物糖化转化乙醇技术研究
DOI：
10.1016/j.renene.2010.06.001
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发表时间：
2011-01-01
期刊：
影响因子：
8.7
通讯作者：
Mou, Haijin
中科院分区：
文献类型：
--
作者：
Ge, Leilei;Wang, Peng;Mou, Haijin
漂浮残渣是海藻酸盐提取过程中的一种剩余副产物，含有大量的纤维素类物质。本文研究了FR作为可再生能源利用的技术可行性。研究了稀硫酸预处理和进一步酶解FR生产酵母可发酵糖（葡萄糖）的工艺。通过使用浓度为0、0.1、0.2、0.5和1.0%（w/v）的硫酸在121 ℃下分别进行0.5、1.0和1.5 h的稀硫酸预处理。酶解体系由纤维素酶和纤维二糖酶组成。结果表明，纤维素含量高（30.0 ± 0.07%），半纤维素含量低（2.2 ± 0.86%），是一种理想的生物质能源。酸预处理通过增加FR的反应表面积来提高纤维素酶和纤维二糖酶的水解效率，从而提高发酵葡萄糖的最终产率。在稀硫酸预处理的最佳条件下（0.1%w/v，121 ℃），葡萄糖的最大得率可达277.5mg/gFR。1.0 h）随后酶水解（50 ° C，pH 4.8，48 h）。在30 ℃条件下发酵36 h，乙醇转化率达到41.2%，相当于理论产率的80.8%。这表明与陆生植物相比，包括FR在内的海藻加工废弃物中的纤维素更容易水解产生葡萄糖。FR作为一种潜在的生产生物乙醇的原料显示了良好的前景。(c)2010爱思唯尔有限公司版权所有。
Floating residue (FR), a surplus by-product from the alginate extraction process, contains large amount of cellulosic materials. The technical feasibility of FR utilization as a resource of renewable energy was investigated in this paper. The production of yeast-fermentable sugars (glucose) from FR was studied by dilute sulfuric acid pretreatment and further enzymatic hydrolysis. Dilute sulfuric acid pretreatment was conducted by using sulfuric acid at concentration of 0, 0.1, 0.2, 0.5 and 1.0%(w/v) for 0.5, 1.0 and 1.5 h respectively at 121 degrees C. The system of enzymatic hydrolysis consisted of cellulase and cellobiase. Results showed that FR might be a perfect bioenergy resource, containing high content of cellulose (30.0 +/- 0.07%) and little hemicellulose (2.2 +/- 0.86%). The acid pretreatment improved the hydrolysis efficiency of cellulase and cellobiase by increasing the reaction surface area of FR and enhanced the final yield of glucose for fermentation. The maximum yield of glucose reached 277.5 mg/g FR under the optimal condition of dilute sulfuric acid pretreatment (0.1% w/v, 121 degrees C. 1.0 h) followed by enzymatic hydrolysis (50 degrees C, pH 4.8, 48 h). After fermentation by Saccharomyces cerevisiae at 30 degrees C for 36 h, the ethanol conversion rate of the concentrated hydrolysates reached 41.2%, which corresponds to 80.8% of the theoretical yield. It indicates that cellulose in seaweed processing wastes including FR is easily hydrolyzed to produce glucose in comparison with that in terrestrial plants. FR shows excellent prospects as a potential feedstock for the production of bioethanol. (c) 2010 Elsevier Ltd. All rights reserved.