Signal-on Protein Detection via Dye Translocation between Aptamer and Quantum Dot
Signal-on Protein Detection via Dye Translocation between Aptamer and Quantum Dot
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DOI：
10.1021/acsami.6b02871
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发表时间：
2016-05-18
影响因子：
9.5
通讯作者：
Chen, Lin-Chi
中科院分区：
文献类型：
--
作者：
Lao, Yeh-Hsing;Chi, Chun-Wei;Chen, Lin-Chi
花青染料和带负电荷的量子点之间的独特相互作用被用于构建用于蛋白质检测和独特的适体表征的信号接通双适体量子点（QD）Forster共振能量转移（FRET）信标。信标包含一对适体，一个插入花青染料（YOYO-3），另一个与带负电荷的羧基-QD缀合。当靶蛋白存在时，两个适体发生结构折叠和夹心缔合。因此，YOYO-3从折叠的适体中被置换并转移到未封闭的QD表面，以产生靶浓度依赖性FRET信号。作为一个证明的原则，我们证明了检测范围从纳摩尔浓度到亚微摩尔浓度的凝血酶，并确认使用圆柱照明共聚焦光谱（CICS）的染料易位。所提出的信标提供了一个简单，快速，信号FRET检测蛋白质，以及一个潜在的平台，不同的适体筛选。
A unique interaction between the cyanine dye and negatively charged quantum dot is used to construct a signal-on biaptameric quantum dot (QD) Forster resonance energy transfer (FRET) beacon for protein detection and distinct aptamer characterization. The beacon comprises a pair of aptamers, one intercalated with the cyanine dye (YOYO-3) and the other conjugated to a negatively charged, carboxyl-QD. When the target protein is present, structural folding and sandwich association of the two aptamers take place. As a consequence, YOYO-3 is displaced from the folded aptamer and transferred to the unblocked QD surface to yield a target concentration-dependent FRET signal. As a proof-of-principle, we demonstrate the detection of thrombin ranging from nanomolar to submicromolar concentrations and confirm the dye translocation using cylindrical illumination confocal spectroscopy (CICS). The proposed beacon provides a simple, rapid, signal-on FRET detection for protein as well as a potential platform for distinct aptamer screening.