Surface Transient Binding-Based Fluorescence Correlation Spectroscopy (STB-FCS), a Simple and Easy-to-Implement Method to Extend the Upper Limit of the Time Window to Seconds
Surface Transient Binding-Based Fluorescence Correlation Spectroscopy (STB-FCS), a Simple and Easy-to-Implement Method to Extend the Upper Limit of the Time Window to Seconds
复制标题
基于表面瞬态结合的荧光相关光谱（STB-FCS），一种简单且易于实施的方法，可将时间窗口上限扩展到秒
DOI：
10.1021/acs.jpcb.8b03476
复制
发表时间：
2018-05-10
影响因子：
3.3
通讯作者：
Chen，Chunlai
中科院分区：
文献类型：
--
作者：
Peng，Sijia;Wang，Wenjuan;Chen，Chunlai
Fluorescence correlation spectroscopy is a powerful single-molecule tool that is able to capture kinetic processes occurring at the nanosecond time scale. However, the upper limit of its time window is restricted by the dwell time of the molecule of interest in the confocal detection volume, which is usually around submilliseconds for a freely diffusing biomolecule. Here, we present a simple and easy-to-implement method, named surface transient binding-based fluorescence correlation spectroscopy (STB-FCS), which extends the upper limit of the time window to seconds. We further demonstrated that STB-FCS enables capture of both intramolecular and intermolecular kinetic processes whose time scales cross several orders of magnitude.
