Presence of IL-4-like molecules in larval excretory-secretory products and crude extracts from Anisakis simplex
Presence of IL-4-like molecules in larval excretory-secretory products and crude extracts from Anisakis simplex
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DOI：
10.1046/j.1365-3083.2001.00905.x
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发表时间：
2001-05-01
影响因子：
3.7
通讯作者：
Rodero, M
中科院分区：
文献类型：
--
作者：
Cuéllar, C;Perteguer, MJ;Rodero, M
异尖线虫病的急性症状是由胃肠道壁的I型过敏反应引起的，特异性免疫球蛋白（IG）E升高。本研究的目的是研究白细胞介素（IL）-4在单纯异尖线虫幼虫抗原中的存在。在A.用ELISA法检测了单纯疱疹病毒。用小鼠IL-4免疫家兔，获得特异性抗体，并进行ELISA检测。单纯抗原获得较高的光密度值，这通过蛋白质印迹分析证实。这些血清与A.当在ELISA中重新测试时，单纯抗原导致70-80%的抗原结合抑制。我们证明了A.单纯抗原与针对脊椎动物IL-4产生的抗体反应。结果支持了A.单纯型与IL-4共享几个表位，这对于人类异丝线虫病中的Th 2应答发展是重要的，其中寄生虫可以调节Th 1-Th 2，通过粘膜炎症控制试图避免幼虫排出而对其自身有利。
Acute symptoms of anisakidosis are caused by a type I allergic reaction in the gastrointestinal wall with elevated specific immunoglobulin (Ig)E. The purpose of this study was to investigate the presence of interleukin (IL)-4 in the larval antigens of Anisakis simplex. We detected concentrations of IL-4 in pg/ml when larval excretory-secretory products and crude extract from A. simplex were investigated by ELISA. Specific antibodies were obtained by immunization of rabbits with mouse IL-4 and tested in ELISA against A. simplex antigens obtaining higher values of optical density, that were confirmed by western blot analysis. The absorption of these sera with A. simplex antigen resulted in a 70-80% inhibition of antigen binding when retested in ELISA. We demonstrated that A. simplex antigens react with antibodies raised against vertebrate IL-4. The results obtained by us support the hypothesis that A. simplex shares several epitopes with IL-4, important for the Th2 response development in human anisnkiasis, where the parasite may modulate the Th1-Th2, dichotomy for its own benefit by mucosal inflammation control in an attempt to avoid the larval expelling.