The use of hirudin as universal anticoagulant in haematology, clinical chemistry and blood grouping
The use of hirudin as universal anticoagulant in haematology, clinical chemistry and blood grouping
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DOI：
10.1515/cclm.2001.204
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发表时间：
2001-12-01
影响因子：
6.8
通讯作者：
Thiel, E
中科院分区：
文献类型：
--
作者：
Menssen, HD;Melber, K;Thiel, E
迄今为止，抗凝剂和诊断测试试剂盒程序之间的不良相互作用阻碍了单个统一采血管的开发。与K-2-EDTA、肝素和其他抗凝剂相反，水蛭素仅最小程度地改变血细胞和溶解的血液成分，因此有资格作为用于诊断目的的通用抗凝剂。对从健康志愿者（n=35）和住院患者（n=45）中获得的水蛭化和常规处理血液进行自动全血细胞计数、临床化学分析物和免疫血液学自动分析。水蛭素（400 ATU/ml，血液）充分抗凝血液用于诊断目的。从K-2-EDTA抗凝血和水蛭素抗凝血中获得的自动全血细胞计数的测量值与从水蛭素抗凝血浆和血清中获得的24种临床化学分析物的测量值显著相关。回归分析显示，全血细胞计数和临床化学检查的结果是可预测的，从水蛭素血的相应测量（P=0.001）。免疫血液学测试和交叉匹配水蛭素和本地血液相同的捐助者给出了相同的结果。单个凝血因子，但不是整体凝血分析物，可以从水蛭素血测量。因此，可以设计一种通用的含水蛭素的采血管，用于血液学、血清学和临床化学分析物的自动化分析。
Undesirable interactions between anticoagulants and diagnostic test kit procedures so far have prevented the development of a single uniform blood sampling tube. Contrary to K-2-EDTA, heparin and other anticoagulants, hirudin only minimally alters blood cells and dissolved blood constituents, thus qualifying as a universal anticoagulant for diagnostic purposes. Automated complete blood counts, automated analyses of clinical chemistry analytes and immunohaematology, were performed from hirudinised and routinely processed blood obtained from healthy volunteers (n=35) and hospitalised patients (n=45). Hirudin (400 ATU/ml, blood) sufficiently anticoagulated blood for diagnostic purposes. The measurements of automated complete blood counts obtained from K-2-EDTA-anticoagulated and hirudinised blood correlated significantly as did the measurements of 24 clinical chemistry analytes from hirudinised plasma and serum. Regression analysis revealed that the results of complete blood counts and clinical chemistry tests were predictable from the respective measurements from hirudinised blood (P=0.001). Immunohaematological tests and cross-matching from hirudinised and native blood of the same donors gave identical results. Single clotting factors, but not global coagulation analytes, could be measured from hirudinised blood. Therefore, a universal hirudin-containing blood sampling tube could be designed for automated analysis of haematological, serological and clinical chemistry analytes.