A cDNA clone highly expressed in ripe banana fruit shows homology to pectate lyases
A cDNA clone highly expressed in ripe banana fruit shows homology to pectate lyases
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DOI：
10.1104/pp.114.3.1071
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发表时间：
1997-07-01
期刊：
影响因子：
7.4
通讯作者：
Prat, S
中科院分区：
文献类型：
--
作者：
DominguezPuigjaner, E;Llop, I;Prat, S
从香蕉跃变型果实的cDNA文库中，通过差异筛选，获得了一个编码果胶酸裂解酶同源蛋白的cDNA克隆（BanI7）。北方分析表明，BanI7的mRNA首先检测到的早期跃变型的水果，达到一个稳定的状态在跃变型的高峰，并在过熟果实下降。通过外源施加乙烯可以在绿色香蕉果实中诱导BanI7转录物的积累。这表明该基因的表达受激素控制，其诱导受成熟开始时乙烯产生快速增加的调节。推导的氨基酸序列与欧文氏菌属花粉和植物病原菌的果胶酸裂解酶具有很高的同源性。与被证明能分解植物细胞壁果胶物质的细菌果胶酸裂解酶的相似性表明，BanI7可能在果实成熟过程中果皮硬度的损失中发挥作用。
A cDNA clone (BanI7), encoding a protein homologous to pectate lyase, has been isolated from a cDNA library from climacteric banana fruit by means of differential screening. Northern analysis showed that BanI7 mRNA is first detected in early climacteric fruit, reaches a steady-state maximum at the climacteric peak, and declines thereafter in overripe fruit. Accumulation of the BanI7 transcript can be induced in green banana fruit by exogenous application of ethylene. This demonstrates that expression of this gene is under hormonal control, its induction being regulated by the rapid increase in ethylene production at the onset of ripening. The deduced amino acid sequence derived from the BanI7 cDNA shares significant identity with pectate lyases from pollen and plant pathogenic bacteria pi the genus Erwinia. Similarity to bacterial pectate lyases that were proven to break down the pectic substances of the plant cell wall suggest that BanI7 might play a role in the loss of mesocarp firmness during fruit ripening.