A novel major quantitative trait locus controlling seed development at low temperature in soybean (Glycine max)
A novel major quantitative trait locus controlling seed development at low temperature in soybean (Glycine max)
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DOI：
10.1007/s00122-009-0996-3
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发表时间：
2009-05-01
影响因子：
5.4
通讯作者：
Funatsuki, Hideyuki
中科院分区：
文献类型：
--
作者：
Ikeda, Tatsuya;Ohnishi, Shizen;Funatsuki, Hideyuki
低温是限制大豆生产的关键环境因素之一。为了阐明大豆耐冷性的遗传基础并筛选出有用的标记，利用常温和低温人工气候环境，以及两个耐冷性对照品种杂交获得的重组自交系，对大豆低温结实力进行了QTL分析。我们发现了一个仅在低温下与种子产量相关的大效应QTL（LOD > 15，r（2）> 0.3）。该QTL定位在连锁群A2上的标记Sat_162附近，以前没有鉴定出耐冷性QTL。耐低温基因型不增加角果数，但保持了角果粒数和单粒重，即低温下种子的发育效率。QTL的作用在异源自交系的分离群体中得到证实，该群体提供了近等基因系。含有QTL的基因组区域也影响节数和荚数，无论温度条件如何，尽管这种影响主要与耐冷性无关。这些结果表明，存在一个新的主要遗传因子，控制种子发育，特别是在低温下。这一发现将有助于分子标记辅助选择和了解生殖器官耐冷性的机制。
Low temperature is among the critical environmental factors that limit soybean production. To elucidate the genetic basis for chilling tolerance and identify useful markers, we conducted quantitative trait loci (QTL) analysis of seed-yielding ability at low temperature in soybean (Glycine max), using artificial climatic environments at usual and low temperatures and recombinant inbred lines derived from a cross between two contrasting cultivars in terms of chilling tolerance. We identified a QTL of a large effect (LOD > 15, r (2) > 0.3) associated with seed-yielding ability only at low temperature. The QTL was mapped near marker Sat_162 on linkage group A2, where no QTL for chilling tolerance has previously been identified. The tolerant genotype did not increase the pod number but maintained the seed number per pod and single seed weight, namely, the efficiency of seed development at low temperature. The effect of the QTL was confirmed in a segregating population of heterogeneous inbred families, which provided near-isogenic lines. The genomic region containing the QTL also influenced the node and pod numbers regardless of temperature condition, although this effect was not primarily associated with chilling tolerance. These results suggest the presence of a new major genetic factor that controls seed development specifically at low temperature. The findings will be useful for marker-assisted selection as well as for understanding of the mechanism underlying chilling tolerance in reproductive organs.