INVITRO CULTURE OF MOUSE PRIMORDIAL GERM-CELLS
INVITRO CULTURE OF MOUSE PRIMORDIAL GERM-CELLS
复制标题
DOI：
10.1016/0014-4827(83)90421-4
复制
发表时间：
1983-01-01
影响因子：
3.7
通讯作者：
MCLAREN, A
中科院分区：
文献类型：
--
作者：
DEFELICI, M;MCLAREN, A
从交配后11 1 2 ~ 16 1 2天的小鼠胎性腺（dpc）中分离生殖细胞，并采用各种方法进行体外培养。从13 - 12 dpc开始，雄性和雌性生殖细胞在37℃下均可存活数天。在培养期间，雌性生殖细胞在减数分裂中的比例增加，减数分裂前期后期出现。因此，性腺环境对减数分裂的进行并不是必需的。男性生殖细胞在体外未进入减数分裂。从性腺1112dpc或1212dpc中分离的生殖细胞在37°C的三种培养系统（培养皿、微试板孔、滴油）中均不能存活；细胞密度、底物和培养基各不相同，并试验了几种添加剂，但没有发现细胞活力的提高。另一方面，在30°C以下，11个12天和12个12天的生殖细胞在体外存活至少一周。它们在培养中没有进入减数分裂，但继续进行有丝分裂增殖。
Germ cells were isolated from mouse fetal gonads 11 1 2− 16 1 2 days post coitum (dpc), and exposed to various methods of in vitro culture. From 13 1 2 dpc onwards, both male and female germ cells survived well at 37° C for several days. During the culture period the proportion of female germ cells in meiosis increased and later stages of meiotic prophase were seen. The gonadal environment is therefore not essential for the progress of meiosis. Male germ cells in vitro did not enter meiosis. Germ cells isolated from gonads 11 1 2 or 12 1 2 dpc did not survive at 37° C in any of the three culture systems used (Petri dishes, microtest plate wells, drops under oil); cell density, substrate and culture medium were varied, and several additives tested, but no improvement in viability was detected. Below 30° C, on the other hand, 11 1 2 and 12 1 2 day germ cells survived in vitro for at least a week. They did not enter meiosis in culture, but continued to undergo mitotic proliferation.