Changes in shell membranes during the development of quail embryos
Changes in shell membranes during the development of quail embryos
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DOI：
10.1093/ps/81.2.246
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发表时间：
2002-02-01
期刊：
影响因子：
4.4
通讯作者：
Saito, H
中科院分区：
文献类型：
--
作者：
Yoshizaki, N;Saito, H
鸡蛋的壳膜就像一个包着蛋白和水的袋子。在它与蛋白的界面处，一层光滑的均匀致密的物质称为界膜，界定了壳膜。本研究旨在探讨鹌鹑胚胎发育过程中的限制膜的变化，生长与不被翻转。卵在39 ℃、60%湿度、赤道面朝下绕长轴自动旋转孵化后，有63%的胚胎孵化，而卵不旋转孵化时，孵化率下降到24%。翻转卵赤道区界膜宽度从孵化第0 ~ 2天的74圈逐渐减小到第10天及以后的35圈。相反，通过壳膜蒸发测量的水渗透性在第0 - 6天从4增加到5 nL/mm（2）/min，在第12天及之后增加到9 nL/mm（2）/min。在静止的卵中，卵下侧的界膜宽度的减小延迟到孵育的第8天。孵化第8天，这一组壳膜的透水性为卵上侧膜透水性的51%。40至44纳米似乎是限制膜的临界宽度，在该宽度处可以发生高的水渗透。还表明蛋白阻碍水渗透通过膜。这些结果表明：（1）可能通过消化未知的试剂，在整个表面上的发育过程中，随着转卵，限制膜变薄，（2）这种变薄加速了水渗透通过膜的速率。
The shell membrane of an avian egg acts as a bag enclosing albumen and water. At its interface with the albumen, a smooth layer of homogeneous, dense material called the limiting membrane demarcates the shell membrane. The present study aimed to investigate changes in the limiting membrane during development of quail embryos that were grown with or without being turned. Sixty-three percent of the embryos were hatched after the eggs were incubated at 39 C and in 60% humidity with automatic rotation around their long axis and with their equatorial side down, whereas the hatch rate decreased to 24% when the eggs were incubated without being turned. The width of the limiting membrane at the equatorial region of turned eggs gradually decreased from 74 run on Days 0 to 2 of incubation to 35 run on Day 10 and thereafter. Conversely, water permeability, measured by evaporation through the shell membrane increased 2 from 4 to 5 nL/mm(2) per min on Days 0 to 6, to 9 nL/mm(2) per min on Day 12 and thereafter. In stationary eggs, the decrease in the width of the limiting membrane on the lower side of eggs was delayed until Day 8 of incubation. The water permeability of the shell membrane in this group was 51% of that of the membrane on the upper side of eggs on Day 8 of incubation. Forty to forty-four nanometers seemed to be the critical width of the limiting membrane at which high water permeation could occur. It was also shown that the albumen hinders water permeation through the membrane. These results show that (1) the limiting membrane is made thin during the development over the whole surface with egg-turning, possibly through digestion of still unknown agents, and (2) this thinning accelerates the rate of water permeation through the membrane.