Far-Red and Near-Infrared Seminaphthofluorophores for Targeted Pancreatic Cancer Imaging.
Far-Red and Near-Infrared Seminaphthofluorophores for Targeted Pancreatic Cancer Imaging.
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DOI：
10.1021/acsomega.6b00403
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发表时间：
2017-01-31
期刊：
影响因子：
4.1
通讯作者：
Strongin RM
中科院分区：
文献类型：
--
作者：
Wang L;Barth CW;Sibrian-Vazquez M;Escobedo JO;Lowry M;Muschler J;Li H;Gibbs SL;Strongin RM
选择性突出显示胰腺癌（PC）组织的分子探针有可能通过选择性突出显示单个PC细胞来改善胰腺导管腺癌（PDAC）边缘评估。在这里，我们报告了一个简单而独特的家庭系统修改的红色和近红外荧光探针，表现出场效应衍生的红移。在全身给药后，十三种探针中的两种分布到正常小鼠胰腺。一种选择性地在PDAC的转基因小鼠模型中积累。该探针表现出细胞内积累，并使可视化的四个层次的结构，包括整个器官，切除的组织，单个细胞，和亚细胞器。与先前报道的小分子探针相反，它具有对PDAC细胞的固有亲和力，因此不需要与任何靶向剂缀合。因此，荧光探针不仅可以促进精确图像引导手术的新策略，还可以用于PC检测，治疗结果监测和基础研究。
Molecular probes that selectively highlight pancreatic cancer (PC) tissue have the potential to improve pancreatic ductal adenocarcinoma (PDAC) margin assessment through the selective highlighting of individual PC cells. Herein, we report a simple and unique family of systematically modified red and near-infrared fluorescent probes that exhibit a field-effect-derived redshift. Two of thirteen probes distributed to the normal mouse pancreas following systemic administration. One selectively accumulated in genetically modified mouse models of PDAC. The probe exhibited intracellular accumulation and enabled visualization of four levels of the structure, including the whole organ, resected tissue, individual cells, and subcellular organelles. In contrast to the small-molecule probes reported previously, it possesses an inherent affinity toward PDAC cells and thus does not require conjugation to any targeting agent. The fluorescent probe can thus promote new strategies not only for precision image-guided surgery, but also for PC detection, monitoring of therapeutic outcomes, and basic research.