A homeobox gene of the Antennapedia class is required for human adult erythropoiesis.
A homeobox gene of the Antennapedia class is required for human adult erythropoiesis.
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触角足类的同源盒基因是成人红细胞生成所必需的。
DOI：
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发表时间：
1993
影响因子：
11.1
通讯作者：
A. Gewirtz
中科院分区：
文献类型：
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作者：
K. Takeshita;J. Bollekens;N. Hijiya;M. Ratajczak;F. Ruddle;A. Gewirtz
在这份报告中，我们调查的同源异型盒基因在成人红细胞生成，红细胞在骨髓中形成的过程中的作用。我们最初确定Hox基因在小鼠红白血病细胞中表达的PCR技术，使用简并引物能够检测大多数已知的基因在Hox簇1-4。鉴定出四种不同的转录本：Hox-3.3、-3.5、-3.6和-4.3。使用针对HOX 3C（Hox-3.3的人同源物）的反义寡核苷酸来研究人红系祖细胞的殖民地形成的效果。寡核苷酸抑制红细胞衍生集落形成单位的形成，但不影响血红蛋白化的大小或程度。更原始的红系爆发形成单位集落或髓系集落不受影响。这些结果表明，Hox-3.3参与祖细胞的红系集落形成单位亚群增殖的早期步骤。
In this report we investigate the role of homeobox genes of the Antennapedia class in adult erythropoiesis, the process by which erythrocytes are formed in the bone marrow. We initially identified Hox genes expressed in mouse erythroleukemia cells by a PCR technique using degenerate primers capable of detecting most of the known genes in Hox clusters 1-4. Four different transcripts, Hox-3.3, -3.5, -3.6, and -4.3 were identified. An antisense oligonucleotide directed against HOX3C (human homologue of Hox-3.3) was used to study the effect of the colony formation by human erythroid progenitor cells. The oligonucleotide inhibited the formation of colony-forming unit erythroid-derived colonies but did not affect the size or degree of hemoglobinization. The more primitive erythroid burst-forming unit colonies or myeloid colonies were not affected. These results show that Hox-3.3 is involved in an early step in the proliferation of the erythroid colony-forming unit subset of progenitor cells.