A combination method for preparing casting and transparent liver specimen-猫眼课题宝

DOI：

10.1002/ca.20969

发表时间：

2010-07

期刊：

Clinical Anatomy

影响因子：

2.4

通讯作者：

中科院分区：

医学4区

文献类型：

作者：

Nan Zheng;Jing Gong;Sheng-Bo Yu;Jianfei Zhang;Wei Tang;Cheng‐Hong Zhang;Jian Bai;Jie Liu;Hong-Jin Sui;Bing-Yi Shi

随着肝脏外科和放射学的发展，对肝内血管和肝节段有更准确的认识是必要的。在此之前，这些领域的研究主要是通过解剖肝脏，制备腐蚀铸造标本，重建肝内血管的三维MSCT图像等。本研究的目的是寻找一种新的标本制备方法，既能显示肝内血管，又能同时显示肝内血管与肝外结构的关系。本研究采用三种不同的技术制备兔肝：(a)腐蚀铸造技术；(b)透明技术；(c)铸造与透明相结合技术。结果显示，C组铸型肝与透明肝结合，既能清晰显示肝内血管，又能清晰显示肝外结构。内部血管与外部结构的关系也清晰可见。综上所述，铸造与透明肝标本的联合制备方法为肝内血管的研究，特别是肝段的应用基础研究开辟了新的途径。中国。动物学报，23:559-562,2010。©2010 Wiley‐Liss, Inc。

With the development of hepatic surgery and radiology, a more accurate understanding of intrahepatic vessels and hepatic segments is necessary. Previously, research in these fields was primarily by means of dissecting livers, preparing corrosion cast specimens, reconstructing three‐dimensional MSCT images of intrahepatic vessels, and so on. The aim of this study was to search for a new specimen preparing method, which could demonstrate intrahepatic vessels and the relationships between internal vessels and external structures of liver simultaneously. Rabbit livers were prepared with three different techniques in this study: (a) corrosion‐cast technique; (b) transparency technique; and (c) combination technique of cast and transparency. The results showed that the combination of casting and transparent liver specimens in group C could demonstrate both the intrahepatic vessels and the external structures clearly. The relationships between the internal vessels and external structures could also be observed clearly. In conclusion, the combination method for preparing casting and transparent liver specimen created a new approach for the research of intrahepatic vessels, especially for applied basic research of hepatic segments. Clin. Anat. 23:559–562, 2010. © 2010 Wiley‐Liss, Inc.

A combination method for preparing casting and transparent liver specimen

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