Assessment of the proliferative capacity of the flavanones 8-prenylnaringenin, 6-(1.1-dimethylallyl)naringenin and naringenin in MCF-7 cells and the rat mammary gland
Assessment of the proliferative capacity of the flavanones 8-prenylnaringenin, 6-(1.1-dimethylallyl)naringenin and naringenin in MCF-7 cells and the rat mammary gland
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DOI：
10.1016/j.mce.2014.05.014
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发表时间：
2014-07-05
影响因子：
4.1
通讯作者：
Kretzschmar, Georg
中科院分区：
文献类型：
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作者：
Helle, Janina;Kraeker, Kristin;Kretzschmar, Georg
8-Prenylnaringenin（8-PN）和naringenin（Nar）是在食品和营养补充剂中发现的植物雌激素，而6-（1.1-二甲基烯丙基）naringenin（6-DMAN）是非洲植物的成分。除了假定的有益效果外，它们还可能促进乳腺癌和子宫内膜癌。因此，我们在体外和体内评估了它们对乳腺的增殖和雌激素样作用。在竞争性雌激素受体（ER）配体结合试验中，8-PN对两种ER均显示出较高的相对结合亲和力，优先选择ER α，并且在供试品中对MCF-7细胞的有丝分裂作用最强。在切除卵巢的年轻成年雌性大鼠中暴露3天后，15 mg/kg 8-PN增加乳腺末端芽（TEB）数量的能力最高，并刺激上皮导管细胞中增殖标记物的表达，其次是6-DMAN和Nar，但总体而言，与17 β-雌二醇（E2）相比，其刺激增殖的能力较弱。(C)由Elsevier爱尔兰Ltd.出版
8-Prenylnaringenin (8-PN) and naringenin (Nar) are phytoestrogens found in food items and nutritional supplements, while 6-(1.1-dimethylallyl)naringenin (6-DMAN) is a component of an African plant. Besides their assumed beneficial effects they may promote mammary and endometrial cancer. We therefore assessed their proliferative and estrogenic potential on the mammary gland in vitro and in vivo. In competitive estrogen receptor (ER) ligand binding assays 8-PN displayed a high relative binding affinity for both ERs with a preference for ER alpha and had the strongest mitotic effect on MCF-7 cells among the test substances. In a three day exposure in young adult ovariectomized female rats 15 mg/kg 8-PN had the highest capacity to increase the number of terminal end buds (TEB) in the mammary gland and stimulated expression of proliferation markers in epithelial ductal cells, followed by 6-DMAN and Nar, but overall their capacity to stimulate proliferation was weak in comparison to 17 beta-Estradiol (E2). (C) 2014 Published by Elsevier Ireland Ltd.