Roles of phosphorylation sites in regulating activity of the transcription factor Pho4
Roles of phosphorylation sites in regulating activity of the transcription factor Pho4
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DOI：
10.1126/science.284.5416.977
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发表时间：
1999-05-07
期刊：
影响因子：
56.9
通讯作者：
O'Shea, EK
中科院分区：
文献类型：
--
作者：
Komeili, A;O'Shea, EK
转录因子通常在多个位点被磷酸化。这里显示出芽酵母转录因子 Pho4 上的多个磷酸化位点在调节该因子的活性中发挥着独特且可分离的作用。 Pho4 在两个位点的磷酸化促进该因子的核输出，而在第三个位点的磷酸化则抑制其核输入。第四个位点的磷酸化可阻断 Pho4 与转录因子 Pho2 的相互作用。多个磷酸化位点提供了重叠且部分冗余的调控层，可有效控制 Pho4 的活性。
Transcription factors are often phosphorylated at multiple sites. Here it is shown that multiple phosphorylation sites on the budding yeast transcription factor Pho4 play distinct and separable roles in regulating the factor's activity. Phosphorylation of Pho4 at two sites promotes the factor's nuclear export and phosphorylation at a third site inhibits its nuclear import. Phosphorylation of a fourth site blocks the interaction of Pho4 with the transcription factor Pho2. Multiple phosphorylation sites provide overlapping and partially redundant Layers of regulation that function to efficiently control the activity of Pho4.