LASER RAMAN-SPECTRA OF CALF THYMUS CHROMATIN AND ITS CONSTITUENTS
LASER RAMAN-SPECTRA OF CALF THYMUS CHROMATIN AND ITS CONSTITUENTS
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DOI：
10.1016/s0006-3495(85)83937-0
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发表时间：
1985-01-01
影响因子：
3.4
通讯作者：
LEWIS, PN
中科院分区：
文献类型：
--
作者：
SAVOIE, R;JUTIER, JJ;LEWIS, PN
对小牛胸腺染色质、核心染色质、(H3.H4)/DNA复合体和分离的DNA进行了广泛的拉曼测量。结果表明，核小体组蛋白的α-螺旋含量逐渐增加，因为它们形成了导致八聚体核小体核心形成的异质复合体。后者的二级结构不会改变，因为它与DNA结合。光谱表明，DNA在核小体中基本上保持了B构象，尽管核糖-磷酸骨架可能发生了轻微的变化。核小体组蛋白和DNA之间没有特异性的相互作用，组蛋白H1可能选择性地与胸腺嘧啶碱基相互作用。
Extensive Raman measurements have been made on calf thymus chromatin, core chromatin, the (H3.H4)/DNA complex, and isolated DNA. The results indicate that the .alpha.-helical content of the nucleosomal histones gradually increases as they form the heterocomplexes that lead to the formation of the octameric nucleosome core. The secondary structure of the latter is not modified as it binds to DNA. The spectra indicate that the DNA essentially retains its B conformation in nucleosomes, although slight changes probably occur in the ribose-phosphate backbone. No specific interactions between the nucleosomal histones and DNA can be established from the spectra, histone H1 possibly interacts selectivity with the thymine bases.