Functional analysis of pneumolysin by use of monoclonal antibodies
Functional analysis of pneumolysin by use of monoclonal antibodies
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DOI：
10.1128/iai.64.2.480-484.1996
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发表时间：
1996-02-01
影响因子：
3.1
通讯作者：
Mitchell, TJ
中科院分区：
文献类型：
--
作者：
delosToyos, JR;Mendez, FJ;Mitchell, TJ
我们已经生产了一组针对肺炎链球菌的膜破坏毒素--肺炎溶血素的单抗。我们已经用这些抗体鉴定了参与该毒素裂解机制的毒素序列的三个区域，其中两个区域可能形成了该毒素的细胞结合部位。针对第三位点的抗体可抑制该毒素的裂解作用，但不能抑制该毒素与细胞的结合。这个位置参与了与细胞膜中形成孔有关的齐聚过程。其中两个表位也存在于相关毒素Perringolysin O中。
We have produced a panel of monoclonal antibodies to pneumolysin, the membrane-damaging toxin from Streptococcus pneumoniae. We have used these antibodies to identify three regions of the toxin sequence that are involved in the lytic mechanism of this toxin, Two of these sites probably form the cell binding site of this toxin. Antibodies to the third site inhibit the lytic action of this toxin but not the binding of this toxin to cells. This site is engaged in the oligomerization process involved in the formation of pores in cell membranes. Two of these epitopes are also present in the related toxin perfringolysin O.