A histopathologic study of the controlling role of T cells on experimental periodontitis in rats
A histopathologic study of the controlling role of T cells on experimental periodontitis in rats
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T细胞对大鼠实验性牙周炎控制作用的组织病理学研究
DOI：
10.1016/j.jds.2017.05.006
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发表时间：
2018
影响因子：
3.5
通讯作者：
Hara Yoshitaka
中科院分区：
文献类型：
--
作者：
Izumi Satoshi;Yoshinaga Yasunori;Nakamura Hirotaka;Takamori Akiko;Takamori Yuzo;Ukai Takashi;Shiraishi Chiaki;Hara Yoshitaka
背景/目的牙周炎的发生和发展与细菌感染和免疫反应有关。T细胞在免疫应答中起作用，据报道在炎性骨丢失中诱导骨吸收。然而，T细胞在牙周破坏中的确切作用仍不清楚。使用我们的实验模型牙周炎，我们的目的是调查的影响，T细胞对牙周destruction.Materials和方法雄性无胸腺裸（Nu）和正常胸腺野生型（WT）大鼠分为免疫（I-Nu和I-WT），非免疫（nI-Nu和nI-WT）。免疫组用脂多糖（LPS）腹腔注射免疫。非免疫组接受磷酸盐缓冲盐水（PBS）。未给药组未给予任何药物。免疫组和非免疫组均于右侧腭部龈沟内涂抹LPS，每日1次，共20 d。组织病理学和组织计量学研究了附着丧失、炎性细胞和破骨细胞的数量以及牙槽骨的水平。破骨细胞用抗酒石酸酸性磷酸酶染色。IL-4阳性细胞的数量进行了评估immunohistologic.ResultsLoss的附着，炎症细胞的数量，牙槽骨的水平，破骨细胞的数量显着增加，在nI-WT组相比，nI-Nu组。然而，与I-WT组相比，I-Nu组的参数显著增加。IL-4阳性细胞的数量是在I-WT组比在I-Nu group.ConclusionT细胞促进炎症在非免疫动物，但是，他们调节这些过程中免疫动物。
Background/purposeThe onset and progression of periodontitis involve bacterial infection and the immune response. T cells function in the immune response and reportedly induce bone resorption in inflammatory bone loss. However, the exact role of T cells in periodontal destruction remains unclear. Using our experimental model of periodontitis, we aimed to investigate the influence of T cells on periodontal destruction.Materials and methodsMale athymic nude (Nu) and euthymic wild-type (WT) rats were divided into the immunized (I-Nu and I-WT), non-immunized (nI-Nu and nI-WT). The immunized groups were immunized intraperitoneally with lipopolysaccharide (LPS). The non-immunized groups received phosphate-buffered saline (PBS). Nothing was administered to the non-treated groups. LPS was applied to the right palatal gingival sulcus in the immunized and non-immunized groups daily for 20 days. Loss of attachment, numbers of inflammatory cells and osteoclasts, and levels of alveolar bone were investigated histopathologically and histometrically. Osteoclasts were stained with tartrate-resistant acid phosphatase. The numbers of IL-4-positive cells were evaluated immunohistologically.ResultsLoss of attachment, numbers of inflammatory cells, levels of alveolar bone, and the number of osteoclasts were significantly increased in the nI-WT group compared with the nI-Nu group. However, the parameters were significantly increased in the I-Nu group compared with the I-WT group. The number of IL-4-positive cells was greater in the I-WT group than in the I-Nu group.ConclusionT cells promote inflammation in non-immunized animals; however, they regulate these processes in immunized animals.