Linkage map and congenic strains to localize blood pressure QTL on rat chromosome 10
Linkage map and congenic strains to localize blood pressure QTL on rat chromosome 10
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DOI：
10.1007/s003359900399
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发表时间：
1997-04-01
期刊：
影响因子：
2.5
通讯作者：
Rapp, JP
中科院分区：
文献类型：
--
作者：
Dukhanina, OI;Dene, H;Rapp, JP
本研究的目的是利用染色体分选的DNA构建大鼠第10号染色体连锁图，并利用该连锁图构建血压QTL定位的一致性品系。连锁作图组由三个F-2群体共418只大鼠组成。32个新的和29个已知的微卫星标记被放置在地图上，跨越88.9厘米摩根（cM）。标记间的平均距离为1.46cM。没有标记物分离超过6.8 cM。通过将来自米兰正常血压菌株（MNS）的Chr 10的各个片段渐渗到Dahl盐敏感（S）菌株的背景上来构建四个同源菌株。当在S背景上表达时，对血压（35 - 42 mm Hg）具有强烈影响的血压QTL定位于D10Mco6和D10Mcol之间的31-cM区域。该区域不包括诱导型一氧化氮合酶（Nos 2）的位点，该位点被认为是QTL的候选位点。
Our purposes were to develop a linkage map for rat Chromosome (Chr) 10, using chromosome-sorted DNA, and to construct con,aenic strains to localize blood pressure quantitative trait loci (QTL) on Chr 10 with the map. The linkage mapping panel consisted of three F-2 populations totaling 418 rats. Thirty-two new and 29 known microsatellite markers were placed on the map, which spanned 88.9 centiMorgans (cM). The average distance between markers was 1.46 cM. No markers were separated by more than 6.8 cM. Four congenic strains were constructed by introgressing various segments of Chr 10 from the Milan normotensive strain (MNS) onto the background of the Dahl salt-sensitive (S) strain. A blood pressure QTL with a strong effect on blood pressure (35-42 mm Hg) when expressed on the S background was localized to a 31-cM region between D10Mco6 and D10Mcol. The region does not include the locus for inducible nitric oxide synthase (Nos2), which had been considered to be a candidate locus for the QTL.