Neurocalcin regulates nighttime sleep and arousal in Drosophila
Neurocalcin regulates nighttime sleep and arousal in Drosophila
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DOI：
10.7554/elife.38114
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发表时间：
2019-03-13
期刊：
影响因子：
7.7
通讯作者：
Jepson, James
中科院分区：
文献类型：
--
作者：
Chen, Ko-Fan;Lowe, Simon;Jepson, James
不同生物体的类睡眠状态可以分为不同的阶段，每个阶段都有一个特征性的唤醒阈值。然而，不同睡眠阶段的分子通路仍不清楚。果蝇（Drosophila melanogaster）在白天和晚上都表现出巩固的睡眠，与白天睡眠相比，夜间睡眠与更高的唤醒阈值相关。在这里，我们确定了神经元钙传感器蛋白Neurocalcin（NCA）的作用，在夜间促进睡眠，但不是白天通过抑制夜间觉醒和多动症。我们表明，昼夜节律和光感通路定义的时间窗口，NCA促进睡眠。此外，我们发现NCA通过抑制分散的唤醒促进神经网络的突触释放来促进睡眠，并证明了蘑菇体（睡眠调节中心）是该网络中的一个模块。我们的研究结果推进了对睡眠阶段如何由基因定义的理解。
Sleep-like states in diverse organisms can be separated into distinct stages, each with a characteristic arousal threshold. However, the molecular pathways underlying different sleep stages remain unclear. The fruit fly, Drosophila melanogaster, exhibits consolidated sleep during both day and night, with night sleep associated with higher arousal thresholds compared to day sleep. Here we identify a role for the neuronal calcium sensor protein Neurocalcin (NCA) in promoting sleep during the night but not the day by suppressing nocturnal arousal and hyperactivity. We show that both circadian and light-sensing pathways define the temporal window in which NCA promotes sleep. Furthermore, we find that NCA promotes sleep by suppressing synaptic release from a dispersed wake-promoting neural network and demonstrate that the mushroom bodies, a sleep-regulatory center, are a module within this network. Our results advance the understanding of how sleep stages are genetically defined.