Abundant self-amplifying intermediate progenitors in the subventricular zone of the Chinese tree shrew neocortex
Abundant self-amplifying intermediate progenitors in the subventricular zone of the Chinese tree shrew neocortex
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中国树鼩新皮质脑室下区丰富的自放大中间祖细胞
DOI：
10.1093/cercor/bhz315
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Yin C;Zhou X;Yao Y;Wang W;Wu Q;Wang X
在进化过程中，脑室下区（SVZ）的神经祖细胞具有基本功能，从脑体积扩张到六层新皮层的产生。在无脑动物模型中，如啮齿动物，SVZ中的大多数神经祖细胞是中间祖细胞（IPC）。啮齿类动物中的大多数IPC都经历神经原性分裂，只有一小部分分裂次数非常有限，以产生一些神经元。与此同时，在回脑动物中，如灵长类动物，IPC能够自我更新多达五个连续的分裂。然而，丰富的IPC连续增殖能力尚未直接观察到在非灵长类物种。在这项研究中，我们研究了神经祖细胞在中国树鼩（树鼩），一种无脑动物与灵长类动物比啮齿类动物更接近的发展。我们确定了SVZ的扩展和新皮质中存在外放射状胶质细胞（oRG）。我们还发现，IPC有能力自我扩增多次，因此作为主要的增殖祖细胞。据我们所知，我们的研究提供了第一个直接的证据，丰富的IPC与增殖潜力的非灵长类动物物种，进一步支持的关键作用，IPC在脑扩展。
During evolution, neural progenitor cells in the subventricular zone (SVZ) have fundamental functions, ranging from brain volume expansion to the generation of a six-layered neocortex. In lissencephalic animal models, such as rodents, the majority of neural progenitors in the SVZ are intermediate progenitor cells (IPCs). Most IPCs in rodents undergo neurogenic division, and only a small portion of them divide a very limited number of times to generate a few neurons. Meanwhile, in gyrencephalic animals, such as primates, IPCs are able to self-renew for up to five successive divisions. However, abundant IPCs with successive proliferative capacity have not been directly observed in nonprimate species. In this study, we examined the development of neural progenitors in the Chinese tree shrew (Tupaia belangeri chinensis), a lissencephalic animal with closer affinity than rodents to primates. We identified an expansion of the SVZ and the presence of outer radial glial (oRG) cells in the neocortex. We also found that IPCs have the capacity to self-amplify multiple times and therefore serve as major proliferative progenitors. To our knowledge, our study provides the first direct evidence of abundant IPCs with proliferative potential in a nonprimate species, further supporting the key role of IPCs in brain expansion.