Atomic-force microscopy: Rhodopsin dimers in native disc membranes
Atomic-force microscopy: Rhodopsin dimers in native disc membranes
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DOI：
10.1038/421127a
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发表时间：
2003-01-09
期刊：
影响因子：
64.8
通讯作者：
Palczewski, K
中科院分区：
文献类型：
--
作者：
Fotiadis, D;Liang, Y;Palczewski, K
在脊椎动物视网膜光感受器中，视杆外节盘膜含有密集的视紫红质分子，用于最佳的光吸收和随后的视觉信号级联放大，但这些光子受体如何相对于彼此组织尚不清楚。在这里，我们使用红外激光原子力显微镜，以揭示天然安排的视紫红质，形成准晶体阵列的二聚体在小鼠椎间盘膜。这些紧密包装的视紫红质二聚体在天然膜的可视化提供了实验支持早期推断其超分子结构，并提供了深入了解光信号是如何控制的。
In vertebrate retinal photoreceptors, the rod outer-segment disc membranes contain densely packed rhodopsin molecules for optimal light absorption and subsequent amplification by the visual signalling cascade, but how these photon receptors are organized with respect to each other is not known. Here we use infrared-laser atomic-force microscopy to reveal the native arrangement of rhodopsin, which forms paracrystalline arrays of dimers in mouse disc membranes. The visualization of these closely packed rhodopsin dimers in native membranes gives experimental support to earlier inferences about their supramolecular structure,and provides insight into how light signalling is controlled.