A new insite into application for barley chromosome addition lines of common wheat : achievement of stigmasterol accumulation
A new insite into application for barley chromosome addition lines of common wheat : achievement of stigmasterol accumulation
复制标题
普通小麦大麦染色体附加系应用的新现场：豆甾醇积累的实现
DOI：
10.1104/pp.111.183533
复制
发表时间：
2011
期刊：
影响因子：
7.4
通讯作者：
他
中科院分区：
文献类型：
--
作者：
Tang J;他
大麦（Hordeum vulgare）的生物活性物质含量远高于小麦（Triticum aestivum）。为了研究加性和（或）增效作用对大麦染色体甾醇含量的影响，我们利用普通小麦正常杂交获得的一系列大麦染色体附加系。在测定2周龄幼苗和干种子中的植物甾醇含量时，我们发现大麦3号染色体附加系（3H）幼苗中的豆甾醇含量比原小麦系和其他大麦染色体附加系高1.5倍，而种子中没有。同时，我们测定了植物甾醇生物合成相关基因在小麦和各附加系幼苗中的总体表达模式，以评估甾醇途径中各基因的相对表达量。由于我们阐明了编码甾醇C-22去饱和酶的cyp710a8（细胞色素P450亚家族）是高水平的豆甾醇的关键特征，因此我们分离了小麦和大麦cyp710a8基因的全长cdna。这些cyp710a8基因在大麦（3H）和小麦（3A， 3B和3D）的3号染色体上定位，cyp710a8基因在3H添加系中表达增加，表明它与污名甾醇积累有关。在拟南芥中过表达cyp710a8基因增加了豆甾醇含量，但不改变总甾醇水平。我们的研究结果为了解普通小麦中生物活性化合物的积累提供了新的见解，并为评估植物代谢谱提供了新的方法。
Barley (Hordeum vulgare) has a much higher content of bioactive substances than wheat (Triticum aestivum). In order to investigate additive and/or synergistic effect(s) on the phytosterol content of barley chromosomes, we used a series of barley chromosome addition lines of common wheat that were produced by normal crossing. In determining the plant sterol levels in 2-week-old seedlings and dry seeds, we found that the level of stigmasterol in the barley chromosome 3 addition (3H) line in the seedlings was 1.5-fold higher than that in the original wheat line and in the other barley chromosome addition lines, but not in the seeds. Simultaneously, we determined the overall expression pattern of genes related to plant sterol biosynthesis in the seedlings of wheat and each addition line to assess the relative expression of each gene in the sterol pathway. Since we elucidated theCYP710A8(cytochrome P450 subfamily)-encoding sterol C-22 desaturase as a key characteristic for the higher level of stigmasterol, full-length cDNAs of wheat and barleyCYP710A8genes were isolated. TheseCYP710A8genes were mapped on chromosome 3 in barley (3H) and wheat (3A, 3B, and 3D), and the expression ofCYP710A8genes increased in the 3H addition line, indicating that it is responsible for stigmasterol accumulation. Overexpression of theCYP710A8genes in Arabidopsis increased the stigmasterol content but did not alter the total sterol level. Our results provide new insight into the accumulation of bioactive compounds in common wheat and a new approach for assessing plant metabolism profiles.