Human U87 astrocytoma cell invasion induced by interaction of βig-h3 with integrin α5β1 involves calpain-2.
Human U87 astrocytoma cell invasion induced by interaction of βig-h3 with integrin α5β1 involves calpain-2.
复制标题
β-H3与整合素α5β1相互作用诱导的人类U87星形细胞瘤细胞侵袭涉及Calpain-2。
DOI：
10.1371/journal.pone.0037297
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Gao GD
中科院分区：
文献类型：
--
作者：
Ma J;Cui W;He SM;Duan YH;Heng LJ;Wang L;Gao GD
已知βig-h3参与多种类型肿瘤的侵袭过程，但其在胶质瘤细胞中的机制尚未完全阐明。利用免疫荧光双染、共聚焦成像分析和免疫共沉淀实验，我们发现βig-h3与整合素α5β1在U87细胞中共定位。我们试图通过进行细胞侵袭试验和明胶酶谱实验来阐明这种相互作用的功能。我们发现siRNA敲低βig-h3和calpain-2会损害细胞侵袭和MMP分泌。此外，已知βig-h3、整合素和钙蛋白酶-2受Ca 2+调节，并且它们也参与肿瘤细胞侵袭。因此，我们进一步研究了calpain-2是否与βig-h3-integrin α5β1相互作用有关，从而影响U87细胞的侵袭。我们的数据表明，βig-h3与整合素α5β1共定位，以增强U87细胞的侵袭力，并且钙蛋白酶2作为下游分子参与了这一过程。
It is known that βig-h3 is involved in the invasive process of many types of tumors, but its mechanism in glioma cells has not been fully clarified. Using immunofluorescent double-staining and confocal imaging analysis, and co-immunoprecipitation assays, we found that βig-h3 co-localized with integrin α5β1 in U87 cells. We sought to elucidate the function of this interaction by performing cell invasion assays and gelatin zymography experiments. We found that siRNA knockdowns of βig-h3 and calpain-2 impaired cell invasion and MMP secretion. Moreover, βig-h3, integrins and calpain-2 are known to be regulated by Ca2+, and they are also involved in tumor cell invasion. Therefore, we further investigated if calpain-2 was relevant to βig-h3-integrin α5β1 interaction to affect U87 cell invasion. Our data showed that βig-h3 co-localized with integrin α5β1 to enhance the invasion of U87 cells, and that calpain-2, is involved in this process, acting as a downstream molecule.
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影响因子：
4.3
作者：
GAO, JX;WILKINS, J;ISSEKUTZ, AC
通讯作者：
ISSEKUTZ, AC
DOI：
10.1016/j.bbamcr.2008.09.019
发表时间：
2009-02-01
影响因子：
5.1
作者：
Martin, Sophie;Cosset, Erika C.;Dontenwill, Monique
通讯作者：
Dontenwill, Monique
DOI：
10.1016/s0006-291x(02)00576-4
发表时间：
2002-06-28
影响因子：
3.1
作者：
Bae, JS;Lee, SH;Kim, IS
通讯作者：
Kim, IS
影响因子：
3.7
作者：
Kawataki, Tomoyuki;Yamane, Tetsu;Patarroyo, Manuel
通讯作者：
Patarroyo, Manuel
影响因子：
4.4
作者：
Kim, Kwang Won;Choi, Chang Hwa;Kim, Yong Keun
通讯作者：
Kim, Yong Keun