TISSUE CULTURE CYTOPATHIC AND PLAQUE ASSAYS FOR MOUSE HEPATITIS VIRUSES
TISSUE CULTURE CYTOPATHIC AND PLAQUE ASSAYS FOR MOUSE HEPATITIS VIRUSES
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DOI：
10.3181/00379727-113-28378
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发表时间：
1963-01-01
影响因子：
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通讯作者：
ROWE, WP
中科院分区：
文献类型：
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作者：
HARTLEY, JW;ROWE, WP
特征性细胞病变的发生和补体结合抗原的产生与几种小鼠肝炎病毒株在含鸡血清培养基中维持的克隆NCTC 1469 C3H小鼠肝细胞中的繁殖相关。该组织培养系统适用于实验室适应和最近分离的病毒株，并允许开发一种快速灵敏的空斑测定方法，该方法对于中和试验和组织培养和小鼠传代细胞系中病毒的定量均令人满意。
Development of a characteristic cytopathic change and production of complement fixing antigen are associated with propagation of several strains of mouse hepatitis viruses in clone NCTC 1469 C3H mouse liver cells maintained in chicken serum-containing medium. This tissue culture system was useful for both laboratory adapted and recently isolated strains of virus and permitted the development of a rapid and sensitive plaque assay method that was satisfactory for neutralization tests and for quantitation of virus in both tissue culture and mouse passage lines.