Sequencing shorter cfDNA fragments improves the fetal DNA fraction in noninvasive prenatal testing
Sequencing shorter cfDNA fragments improves the fetal DNA fraction in noninvasive prenatal testing
复制标题
对较短的 cfDNA 片段进行测序可提高无创产前检测中的胎儿 DNA 分数
DOI：
10.1016/j.ajog.2019.05.023
复制
发表时间：
2019-10-01
影响因子：
9.8
通讯作者：
Wang, Ting
中科院分区：
文献类型：
--
作者：
Qiao, Longwei;Yu, Bin;Wang, Ting
背景：对孕妇血浆中的无细胞DNA进行测序是一种有效的非侵入性产前检测技术，已在全球范围内用于胎儿非整倍体筛查。但由于胎儿比例较低(10%)，其临床应用受到限制。结论：对较短的无细胞DNA片段进行测序是一种合理的策略，可以减少因胎儿比例较低而导致无呼叫结果的概率，值得推荐给孕妇。
BACKGROUND: Sequencing cell-free DNA in maternal plasma is an effective noninvasive prenatal testing technique that has been used in fetal aneuploidy screening worldwide. However, its clinical application is limited by the low fetal fraction (10%. Improved detection accuracy was also achieved.CONCLUSION: Sequencing shorter cell-free DNA fragments is a reasonable strategy to reduce the probability of no calls results because of low fetal fraction and should be recommended to pregnant subjects.