Production of endothelin-1 and big endothelin-1 by human cardiac myxoma cells - Implications for the origin of myxomas
Production of endothelin-1 and big endothelin-1 by human cardiac myxoma cells - Implications for the origin of myxomas
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DOI：
10.1253/circj.68.1230
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发表时间：
2004-12-01
影响因子：
3.3
通讯作者：
Kurabayashi, M
中科院分区：
文献类型：
--
作者：
Sakamoto, H;Sakamaki, T;Kurabayashi, M
背景虽然心脏粘液瘤的起源仍有争议，但主要的两种假说是肿瘤细胞来源于多潜能间充质细胞或来源于内皮神经组织。方法和结果用酶联免疫吸附试验检测了2株人心脏粘液瘤细胞系中多种细胞因子的产生。培养7天后，在两种粘液瘤细胞系的培养基中检测到极高浓度的白细胞介素-6。在两种粘液瘤细胞系中均观察到CXC趋化因子、白细胞介素-8和生长相关癌基因-a的产生增加。内皮素（ET）-1及其前体，大ET-1，在粘液瘤细胞系的培养基中检测。粘液瘤细胞产生ET-1和大ET-1的能力均高于人脐静脉内皮细胞。与内皮细胞相似，粘液瘤细胞不产生干细胞因子、粒细胞集落刺激因子、肝细胞生长因子和ET-3。结论心脏粘液瘤细胞与内皮细胞产生细胞因子的模式相似，支持肿瘤细胞起源于具有内皮分化能力的间充质细胞的假说。CXC趋化因子的过度产生可能部分解释了组织学良性粘液瘤的恶性潜力。
Background Although the origin of cardiac myxomas is still controversial, the 2 main hypotheses are that the tumor cells originate either from multipotential mesenchymal cells or from endocardial neural tissue.Methods and Results The production of various cytokines in 2 human cardiac myxoma cell lines was examined by enzyme-linked immunosorbent assay. After 7 days of culture, extremely high concentrations of interleukin-6 were detected in the culture media from both myxoma cell lines. Increased production of CXC chemokines, interleukin-8 and growth-related oncogene-a, were observed in both myxoma cell lines. Endothelin (ET)-1 and its precursor, big ET-1, were detected in the culture media from both myxoma cell lines. The production of both ET-1 and big ET-1 by myxoma cells was higher than by human umbilical vein endothelial cells. Similar to endothelial cells, myxoma cells did not produce stem cell factor, granulocyte colony-stimulating factor, hepatocyte growth factor, or ET-3.Conclusions The similarity of the cytokine production pattern between cardiac myxoma cells and endothelial cells supports the hypothesis that the tumor cells originate from mesenchymal cells capable of endothelial differentiation. Overproduction of CXC chemokines may explain, in part, the malignant potential of histologically benign myxomas.