Isolation of multipotent mesenchymal stem cells from umbilical cord blood
Isolation of multipotent mesenchymal stem cells from umbilical cord blood
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DOI：
10.1182/blood-2003-05-1670
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发表时间：
2004-03-01
期刊：
影响因子：
20.3
通讯作者：
Chen, TH
中科院分区：
文献类型：
--
作者：
Lee, OK;Kuo, TK;Chen, TH
脐带血是造血干细胞的重要来源。然而，脐带血是否可以作为间充质干细胞的来源存在争议，间充质干细胞可以分化为不同结缔组织谱系的细胞，如骨，软骨和脂肪，并且在文献中几乎没有关于从脐带血中分离此类细胞的成功报道。在这里，我们报告了一种新的方法来获得单细胞来源的，克隆扩增的间充质干细胞，是多谱系分化潜力的负免疫选择和有限稀释。这些克隆扩增细胞的免疫表型与骨髓间充质干细胞的免疫表型一致。在适当的诱导条件下，这些细胞可以分化成骨、软骨和脂肪。令人惊讶的是，这些细胞也能够在适当的诱导条件下分化成神经胶质细胞样细胞和肝细胞样细胞，因此，这些细胞可能不仅仅是间充质干细胞，如它们分化成所有3个胚层的细胞类型的能力所证明的。总之，脐带血确实含有间充质干细胞，不应被视为医疗废物。它可以作为骨髓间充质干细胞的替代来源。(C)2004年，美国血液学会。
It is well accepted that umbilical cord blood has been a source for hematopoietic stem cells. However, controversy exists as to whether cord blood can serve as a source of mesenchymal stem cells, which can differentiate into cells of different connective tissue lineages such as bone, cartilage, and fat, and little success has been reported in the literature about the isolation of such cells from cord blood. Here we report a novel method to obtain single cell-derived, clonally expanded mesenchymal stem cells that are of multilineage differentiation potential by negative immunoselection and limiting dilution. The immunophenotype of these clonally expanded cells is consistent with that reported for bone marrow mesenchymal stem cells. Under appropriate induction conditions, these cells can differentiate into bone, cartilage, and fat. Surprisingly, these cells were also able to differentiate into neuroglial- and hepatocyte-like cells under appropriate induction conditions and, thus, these cells may be more than mesenchymal stem cells as evidenced by their ability to differentiate into cell types of all 3 germ layers. In conclusion, umbilical cord blood does contain mesenchymal stem cells and should not be regarded as medical waste. It can serve as an alternative source of mesenchymal stem cells to bone marrow. (C) 2004 by The American Society of Hematology.