Phosphate removal and recovery using immobilized phosphate binding proteins
Phosphate removal and recovery using immobilized phosphate binding proteins
复制标题
使用固定化磷酸盐结合蛋白去除和回收磷酸盐
DOI：
10.1016/j.wroa.2018.09.003
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发表时间：
2018
期刊：
影响因子：
7.5
通讯作者：
Mayer, Brooke K.
中科院分区：
文献类型：
--
作者：
Venkiteshwaran, Kaushik;Pokhrel, Nilisha;Hussein, Faten;Antony, Edwin;Mayer, Brooke K.
为了实现磷的循环经济，需要开发创新的水处理系统，这些系统可以可逆地将无机磷酸盐（Pi）去除到超低水平（<100 μg L−1），然后回收Pi用于再利用。本研究利用大肠杆菌磷酸结合蛋白（PBP）作为可重复使用的Pibio-adsorbent，研究了一种新方法。PBP表达，提取，纯化和固定在NHS活化的琼脂糖凝胶珠。将所得PBP珠用Pi饱和并暴露于不同的pH（pH 4.7至12.5）和温度（25-45 °C）以诱导Pi释放。温度从25 °C增加到45 °C，pH值在4.7和8.5之间，释放的吸附Pi小于20%。而在pH11.4和12.5时，吸附的PI分别有62%和86%被释放。动力学实验表明，Pidesorption发生几乎瞬间（<5分钟），无论pH值条件，这是有利的Pirecovery。此外，当PBP珠分别暴露于使用中性和高pH（≥12.5）洗涤的10个重复的吸附/解吸循环时，未观察到Piadsorption或解吸能力的损失。使用PBP珠的最高平均Piadsorption为83 ± 5%，在室温下使用pH 12.5洗涤10个洗涤循环的平均解吸为89 ± 4.1%。PBP的热位移测定表明，蛋白质在10个循环后结构稳定，在pH 4和12.5之间具有统计学上相似的熔解温度。这些结果表明，固定化高亲和力PBP有可能成为一种有效的和可逆的生物吸附剂，适用于从水/废水中回收PiR。
Progress towards a more circular phosphorus economy necessitates development of innovative water treatment systems which can reversibly remove inorganic phosphate (Pi) to ultra-low levels (<100 μg L−1), and subsequently recover the Pifor reuse. In this study, a novel approach using the high-affinityE. coliphosphate binding protein (PBP) as a reusable Pibio-adsorbent was investigated. PBP was expressed, extracted, purified and immobilized on NHS-activated Sepharose beads. The resultant PBP beads were saturated with Piand exposed to varying pH (pH 4.7 to 12.5) and temperatures (25–45 °C) to induce Pirelease. Increase in temperature from 25 to 45 °C and pH conditions between 4.7 and 8.5 released less than 20% of adsorbed Pi. However, 62% and 86% of the adsorbed Piwas released at pH 11.4 and 12.5, respectively. Kinetic experiments showed that Pidesorption occurred nearly instantaneously (<5 min), regardless of pH conditions, which is advantageous for Pirecovery. Additionally, no loss in Piadsorption or desorption capacity was observed when the PBP beads were exposed to 10 repeated cycles of adsorption/desorption using neutral and high pH (≥12.5) washes, respectively. The highest average Piadsorption using the PBP beads was 83 ± 5%, with 89 ± 4.1% average desorption using pH 12.5 washes over 10 wash cycles at room temperature. Thermal shift assay of the PBP showed that the protein was structurally stable after 10 cycles, with statistically similar melting temperatures between pH 4 and 12.5. These results indicate that immobilized high-affinity PBP has the potential to be an effective and reversible bio-adsorbent suitable for Pirecovery from water/wastewater.
影响因子：
2.9
作者：
Solscheid C;Kunzelmann S;Davis CT;Hunter JL;Nofer A;Webb MR
通讯作者：
Webb MR