Light-induced fluorescence for pulpal diagnosis
Light-induced fluorescence for pulpal diagnosis
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用于牙髓诊断的光诱导荧光
DOI：
10.1117/12.424495
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
P. Wilder
中科院分区：
文献类型：
--
作者：
A. Ebihara;L. Liaw;T. Krasieva;P. Wilder
牙髓诊断的直接非组织学方法在临床实践中仍然难以实现。临床活力测试仍然仅限于电、热标准或激光多普勒血流测定法。这些研究的目的是确定使用光诱导荧光作为牙髓评估的非侵入性方式的可行性。例如，这种能力将允许扩大使用髓切断术/牙髓切除术技术。本研究使用临床健康和患病的人类摘除牙髓组织。切除后，将它们快速冷冻并制备标准冷冻切片。使用光谱分析进行组织激发/发射特性的测量。然后使用低光荧光显微镜系统在最佳激发/检测参数下对自发荧光定位和强度进行成像。本研究中使用的激发/检测参数包括 405/605、405/635、405/670、440/550 和 440/635。在最佳参数下，健康组织中的自发荧光强度明显强于患病组织中的自发荧光强度。据推测，自发荧光特征与牙髓中病理相关的结构变化有关。该工作为进一步研究自发荧光、组织学和临床症状之间的关系提供了基础。
A direct non-histological means of pulpal diagnosis remains elusive to clinical practice. Clinical vitality testing remains limited to electric, thermal criteria, or laser Doppler flowmetry. The goal of these investigations was to determine the feasibility of using light-induced fluorescence as a non-invasive modality for pulpal evaluation. Such a capability would, for example, permit expanded use of pulpotomy/pulpectomy techniques. Clinically healthy and diseased human extirpated pulpal tissues were used in this study. After excision, they were rapidly frozen and standard cryosections prepared. Measurement of tissue excitation/emission characteristics was performed using spectrographic analysis. A low-light level fluorescence microscopy system was then used to image autofluorescence localization and intensity at optimal excitation/detection parameters. Excitation/detection parameters used in this study included 405/605, 405/635, 405/670, 440/550, and 440/635. Autofluorescence intensities in healthy tissues were significantly stronger than those in diseased tissues at optimal parameters. It is postulated that autofluorescence characteristics are related to pathology- related structural changes in the pulp. This work provides the basis for further investigation into the relation between autofluorescence, histology and clinical symptoms.