Progressive restriction of cell fates in relation to neuroepithelial cell mingling in the mouse cerebellum
Progressive restriction of cell fates in relation to neuroepithelial cell mingling in the mouse cerebellum
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DOI：
10.1016/s0012-1606(03)00098-8
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发表时间：
2003-06-01
影响因子：
2.7
通讯作者：
Nicolas, JF
中科院分区：
文献类型：
--
作者：
Mathis, L;Nicolas, JF
小脑中的神经发生是通过两个独立的上皮细胞、脑室区 (VZ) 和外部颗粒细胞层 (EGL) 的一系列时间细胞生成进行的。在转基因小鼠中使用 laacZ 细胞谱系示踪剂，我们描述了其出生日期跨越小脑发育的整个时期的细胞克隆，并推断出导致小脑中细胞最终分配的细胞分散序列。可能在神经管形成早期标记的克隆表明，单个祖细胞可以产生所有小脑细胞类型。这些克隆中克隆相关颗粒细胞的分布表明在 EGL 祖细胞分配到小脑之前已经建立了 EGL 祖细胞的中外侧组织。仅限于小脑 VZ 的克隆表明，VZ 衍生出深部核团和多能皮质祖细胞的祖细胞，当小脑 VZ 中的纵向细胞混合变得更加有限时，这些祖细胞就会失去其系统谱系关系。小克隆还表明，细胞分散在内部颗粒层中是径向的，而在分子层中是切向的。总之，这些数据证明了从神经管阶段到成人小脑的细胞相对顺序的广泛维持。 (C) 2003 年爱思唯尔科学（美国）。版权所有。
Neurogenesis in the cerebellum proceeds through a temporal series of cell production from two separate epithelia, the ventricular zone (VZ) and the external granule cell layer (EGL). Using the laacZ cell lineage tracer in transgenic mice, we describe cellular clones whose dates of birth span the entire period of cerebellar development and deduce a sequence of cell dispersion leading to the final allocation of cells in the cerebellum. Clones probably labeled early during neural tube formation show that individual progenitors can give rise to all cerebellar cell types. The distribution of clonally related granule cells in these clones indicates a mediolateral organization of EGL progenitors already established before the allocation of the EGL progenitors to the cerebellum. Clones restricted to the cerebellar VZ show that the VZ derives progenitors for deep nuclei and multipotent cortical progenitors, which lose their systematic lineage relationship when longitudinal cell intermingling in the cerebellar VZ becomes more limited. The small clones also show that cell dispersion is radial in the internal granule layer and tangential in the molecular layer. Together, the data demonstrate the broad maintenance of the relative order of cells from neural tube stages to the adult cerebellum. (C) 2003 Elsevier Science (USA). All rights reserved.