Development and Evaluation of an Enzyme-Linked Immunosorbent Assay with Recombinant SAG2 for Diagnosis of Toxoplasma gondii Infection in Cats
Development and Evaluation of an Enzyme-Linked Immunosorbent Assay with Recombinant SAG2 for Diagnosis of Toxoplasma gondii Infection in Cats
复制标题
使用重组 SAG2 诊断猫弓形虫感染的酶联免疫吸附测定的开发和评估
作者：
Xiaohong Huang;X. Xuan;E. Kimbita;B. Battur;T. Miyazawa;S. Fukumoto;M. Mishima;L. Makala;Hiroshi Suzuki;C. Sugimoto;H. Nagasawa;K. Fujisaki;T. Mikami;I. Igarashi
猫是刚地弓形虫传播的关键。为了建立一种灵敏、特异的猫弓形虫病血清诊断方法，在大肠杆菌中表达弓形虫表面抗原2 (SAG2)，并采用酶联免疫吸附试验（ELISA）评价其诊断潜力。重组SAG2 （rSAG2）酶联免疫吸附试验能够很好地区分刚地弓形虫感染猫血清和正常猫血清。采用ELISA对日本家猫血清样本进行调查，并将结果与市售乳胶凝集试验（LAT）试剂盒的结果进行比较。192份样本中，42份（21.9%）ELISA阳性。42份elisa阳性样本中，LAT阳性39份。ELISA与LAT滴度有显著相关性。150份elisa阴性样本均为LAT阴性。结果表明，利用大肠杆菌表达的rSAG2酶联免疫吸附试验可作为检测猫弓形虫感染的有效方法。
Cats are pivotal in the transmission of Toxoplasma gondii. To develop a sensitive and specific serodiagnostic method for feline toxoplasmosis, surface antigen 2 (SAG2) of T. gondii was expressed in Escherichia coli and its diagnostic potential evaluated in an enzyme-linked immunosorbent assay (ELISA). The ELISA with recombinant SAG2 (rSAG2) was able to differentiate very clearly between sera from cats experimentally infected with T. gondii and sera from normal cats. Serum samples collected from domestic cats in Japan were investigated by the ELISA, and the results were compared with those of a commercially available latex agglutination test (LAT) kit. Of the 192 samples screened, 42 (21.9%) were positive by ELISA. Among the 42 ELISA-positive samples, 39 were positive by LAT. There was a significant correlation between ELISA and LAT titers. All the 150 ELISA-negative samples were negative by LAT. These results indicate that the ELISA with rSAG2 expressed in E. coli should be a useful method for detection of T. gondii infection in cats.