Anisotropicity and flexibility in trilayered microfibrous substrates promote heart valve leaflet tissue engineering.
Anisotropicity and flexibility in trilayered microfibrous substrates promote heart valve leaflet tissue engineering.
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DOI：
10.1088/1748-605x/ac94ae
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发表时间：
2022-10-07
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具有天然三层和各向异性结构的心脏瓣膜小叶基底对于成功的心脏瓣膜组织工程至关重要。在这项研究中，我们使用静电纺丝技术生产三层微纤维瓣叶基板使用两种生物相容性和可生物降解的聚合物-聚（L-乳酸）（PLLA）和聚己内酯（PCL），分别。每层的聚合物浓度不同，使基板的机械和结构的各向异性程度高。PCL瓣叶基材的单向拉伸性能低于PLLA瓣叶基材。然而，PLLA基板表现出比PCL基板更低的弯曲模量。将这些基质与猪瓣膜间质细胞（PVIC）接种，并在静态条件下培养一个月。两种基质都表现出细胞粘附和增殖，导致组织工程构建体的产生。PLLA组织工程化构建体比PCL组织工程化构建体具有更多的细胞生长。PLLA基板表现出更高的亲水性，较低的结晶度，和更显着的各向异性比PCL基板，这可能增强了它们与PVIC的相互作用。基因表达分析显示，PLLA组织工程构建体中α-SMA和1型胶原表达高于PCL组织工程构建体。在各向异性和弯曲性能的差异可能占在这两个单独的聚合物基板中的不同的细胞行为。
Heart valve leaflet substrates with native trilayer and anisotropic structures are crucial for successful heart valve tissue engineering. In this study, we used the electrospinning technique to produce trilayer microfibrous leaflet substrates using two biocompatible and biodegradable polymers - poly (L-lactic acid) (PLLA) and polycaprolactone (PCL), separately. Different polymer concentrations for each layer were applied to bring a high degree of mechanical and structural anisotropy to the substrates. PCL leaflet substrates exhibited lower unidirectional tensile properties than PLLA leaflet substrates. However, the PLLA substrates exhibited a lower flexural modulus than the PCL substrates. These substrates were seeded with porcine valvular interstitial cells (PVICs) and cultured for one month in static conditions. Both substrates exhibited cellular adhesion and proliferation, resulting in the production of tissue-engineered constructs. The PLLA tissue-engineered constructs had more cellular growth than the PCL tissue-engineered constructs. The PLLA substrates showed higher hydrophilicity, lower crystallinity, and more significant anisotropy than PCL substrates, which may have enhanced their interactions with PVICs. Analysis of gene expression showed higher α-SMA and collagen type 1 expression in PLLA tissue-engineered constructs than in PCL tissue-engineered constructs. The differences in anisotropic and flexural properties may have accounted for the different cellular behaviors in these two individual polymer substrates.