Virulence strategies of an insect herbivore and oomycete plant pathogen converge on host E3 SUMO ligase SIZ1.
Virulence strategies of an insect herbivore and oomycete plant pathogen converge on host E3 SUMO ligase SIZ1.
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DOI：
10.1111/nph.18184
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发表时间：
2022-08
期刊：
影响因子：
9.4
通讯作者：
Bos, Jorunn I. B.
中科院分区：
文献类型：
--
作者：
Liu, Shan;Lenoir, Camille J. G.;Amaro, Tiago M. M. M.;Rodriguez, Patricia A.;Huitema, Edgar;Bos, Jorunn I. B.
病原体和害虫分泌蛋白质（效应物）通过改变宿主靶功能和破坏免疫信号网络来干扰植物免疫。病原体和植食性昆虫毒力策略之间的趋同程度在很大程度上是未探索的。我们发现卵菌病原体辣椒疫霉和主要蚜虫害虫桃蚜的效应子靶向宿主免疫调节剂SIZ 1，一种E3 SUMO连接酶。我们在本氏烟草和拟南芥突变体中使用瞬时表达测定来进一步表征效应子-SIZ 1相互作用在植物中的生物学作用。我们表明，卵菌和蚜虫效应，这两个有助于毒力，功能不同的活动对SIZ 1。而M.辣椒疫霉效应子CRN83_152在体内增强SIZ 1-E3 SUMO连接酶活性。SIZ 1有助于宿主对蚜虫和卵菌病原体的易感性。敲除拟南芥SIZ 1基因可降低对蚜虫的易感性，与SNC 1、PAD 4和EDS 1无关。类似地，本氏烟草中SIZ 1的敲低导致辣椒疫霉感染减少。我们的研究结果表明，不同的病原体和害虫的毒力E3 SUMO连接酶的策略，以提高主机的敏感性收敛。
Pathogens and pests secrete proteins (effectors) to interfere with plant immunity through modification of host target functions and disruption of immune signalling networks. The extent of convergence between pathogen and herbivorous insect virulence strategies is largely unexplored. We found that effectors from the oomycete pathogen, Phytophthora capsici, and the major aphid pest, Myzus persicae target the host immune regulator SIZ1, an E3 SUMO ligase. We used transient expression assays in Nicotiana benthamiana as well as Arabidopsis mutants to further characterize biological role of effector–SIZ1 interactions in planta. We show that the oomycete and aphid effector, which both contribute to virulence, feature different activities towards SIZ1. While M. persicae effector Mp64 increases SIZ1 protein levels in transient assays, P. capsici effector CRN83_152 enhances SIZ1‐E3 SUMO ligase activity in vivo. SIZ1 contributes to host susceptibility to aphids and an oomycete pathogen. Knockout of SIZ1 in Arabidopsis decreased susceptibility to aphids, independent of SNC1, PAD4 and EDS1. Similarly SIZ1 knockdown in N. benthamiana led to reduced P. capsici infection. Our results suggest convergence of distinct pathogen and pest virulence strategies on an E3 SUMO ligase to enhance host susceptibility.
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影响因子：
4.5
作者：
Hammoudi V;Fokkens L;Beerens B;Vlachakis G;Chatterjee S;Arroyo-Mateos M;Wackers PFK;Jonker MJ;van den Burg HA
通讯作者：
van den Burg HA
影响因子：
56.9
作者：
DELANEY, TP;UKNES, S;RYALS, J
通讯作者：
RYALS, J
DOI：
10.1111/j.1365-313x.2007.03359.x
发表时间：
2008-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Jin JB;Jin YH;Lee J;Miura K;Yoo CY;Kim WY;Van Oosten M;Hyun Y;Somers DE;Lee I;Yun DJ;Bressan RA;Hasegawa PM
通讯作者：
Hasegawa PM
影响因子：
7.4
作者：
Cheong, Mi Sun;Park, Hyeong Cheol;Yun, Dae-Jin
通讯作者：
Yun, Dae-Jin
影响因子：
11.6
作者：
Catala, Rafael;Ouyang, Jian;Chua, Nam-Hai
通讯作者：
Chua, Nam-Hai