Transfer of simple sequence repeat (SSR) markers from major cereal crops to minor grass species for germplasm characterization and evaluation
Transfer of simple sequence repeat (SSR) markers from major cereal crops to minor grass species for germplasm characterization and evaluation
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DOI：
10.1079/pgr200461
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发表时间：
2005-04
期刊：
影响因子：
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通讯作者：
M. Wang;N. Barkley;Jianming Yu;R. Dean;M. Newman;M. Sorrells;G. Pederson
中科院分区：
文献类型：
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作者：
M. Wang;N. Barkley;Jianming Yu;R. Dean;M. Newman;M. Sorrells;G. Pederson
缺乏足够的DNA标记是对禾本科小种种质资源进行分子鉴定和评价的主要挑战。利用主要谷类作物（自花小麦和水稻，主要是自花高粱和异交玉米）的210个SSR标记，评价了它们在次要禾本科植物（谷子、Eleusine coracana、滨海雀稗、paspalum vaginatum和百草、Cynodon dactylon）上的可转移性。共鉴定出412个跨物种多态性扩增子。超过一半的引物产生可复制的跨种或跨属扩增子。SSR标记的转移率与这些物种的系统发育关系（或遗传亲缘关系）相关。基因组SSR标记的平均转移率与表达序列标签(EST)-SSR标记的平均转移率不同。种间多态性水平（67%）显著高于种内多态性水平（34%），且与种间异交程度有关。自交不亲和种的多态性为57%，异交种的多态性为39%，自花授粉种的多态性为20%。基因组SSRs检测到的多态性水平高于EST-SSRs。讨论了转移多态性SSR标记在种质鉴定和评价中的应用。
A major challenge for the molecular characterization and evaluation of minor grass species germplasm is the lack of sufficient DNA markers. A set of 210 simple sequence repeat (SSR) markers developed from major cereal crops (self-pollinated wheat and rice, mainly self-pollinated sorghum and out-crossing maize) were evaluated for their transferability to minor grass species (finger millet, Eleusine coracana; seashore paspalum, Paspalum vaginatum; and bermudagrass, Cynodon dactylon). In total, 412 cross-species polymorphic amplicons were identified. Over half of the primers generated reproducible cross-species or cross-genus amplicons. The transfer rate of SSR markers was correlated with the phylogenetic relationship (or genetic relatedness) of these species. The average transfer rate of genomic SSR markers was different from the average transfer rate of expressed sequence tag (EST)-SSR markers. The level of polymorphism was significantly higher among species (67%) than within species (34%), and was related to the degree of out-crossing for each species. The level of polymorphism detected within species was 57% from self-incompatible species, 39% from out-crossing species and 20% from self-pollinated species. Genomic SSRs detected a higher level of polymorphism than EST-SSRs. The use of transferred polymorphic SSR markers for the characterization and evaluation of germplasm is discussed.