Proliferation characteristics of the intracellular microsporidian pathogen Nosema bombycis in congenitally infected embryos
Proliferation characteristics of the intracellular microsporidian pathogen Nosema bombycis in congenitally infected embryos
复制标题
细胞内微孢子虫病原菌家蚕微孢子虫在先天性感染胚胎中的增殖特征
DOI：
10.1016/j.jip.2019.107310
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发表时间：
2020
影响因子：
3.4
通讯作者：
Zhou Zeyang
中科院分区：
文献类型：
--
作者：
Song Yue;Tang Yunlin;Yang Qiong;Li Tangxin;He Zhangshuai;Wu Yujiao;He Qiang;Li Tian;Li Chunfeng;Long Mengxian;Chen Jie;Wei Junhong;Bao Jialing;Shen Zigang;Meng Xianzhi;Pan Guoqing;Zhou Zeyang
家蚕微孢子虫（Nosema bombycis）是一种专性胞内病原体，可从受感染的雌虫垂直传播至卵，导致胚胎的先天性感染。本实验研究了N.使用组织病理学方法和深度RNA测序，对家蚕胚胎进行家蚕毒素处理。我们发现N。在胚胎发育早期，即产卵后1-2天，家蚕主要在卵黄颗粒周围增殖。在4-6 dpo时，N.在胚胎附近的不同阶段的家蚕寄生虫被包装到新形成的肠腔中，而剩余的寄生虫继续在卵黄颗粒周围增殖。在新孵化的幼虫（9 dpo），新形成的孢子积累在肠腔中，并立即通过粪便释放到环境中。N. bombycis进一步证实了N. bombycis在胚胎发育的每一个阶段。此外，孢子壁蛋白和极管蛋白的转录水平从4dpo开始增加，表明成熟孢子的活跃形成。两者合计，我们的研究结果提供了一个表征这种细胞内微孢子虫病原体在先天性感染胚胎中的增殖导致垂直传播。
Nosema bombycisis an obligate intracellular pathogen that can be transmitted vertically from infected females to eggs, resulting in congenital infections in embryos. Here we investigated the proliferation characteristics ofN. bombycisin silkworm embryos using a histopathological approach and deep RNA sequencing. We found thatN. bombycisproliferated mainly around yolk granules at the early stage of the embryonic development, 1–2 days post oviposition (dpo). At 4–6 dpo, a portion ofN. bombycisin different stages adjacent to the embryo were packaged into the newly formed intestinal lumen, while the remaining parasites continued to proliferate around yolk granules. In the newly hatched larvae (9 dpo), the newly formed spores accumulated in the gut lumen and immediately were released into the environment via the faeces. Transcriptional profiling ofN. bombycisfurther confirmed multiplication ofN. bombycisthroughout every stage of embryonic development. Additionally, the increased transcriptional level of spore wall proteins and polar tube proteins from 4 dpo indicated an active formation of mature spores. Taken together, our results have provided a characterization of the proliferation of this intracellular microsporidian pathogen in congenitally infected embryos leading to vertical transmission.