Modulation of HIV-like particle assembly in vitro by inositol phosphates
Modulation of HIV-like particle assembly in vitro by inositol phosphates
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DOI：
10.1073/pnas.191224698
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发表时间：
2001-09-11
影响因子：
11.1
通讯作者：
Rein, A
中科院分区：
文献类型：
--
作者：
Campbell, S;Fisher, RJ;Rein, A
HIV-1 Gag蛋白在哺乳动物细胞中组装成直径为100至120 nm的颗粒。重组HIV-1 Gag蛋白在体外完全确定的系统中组装成直径仅为25-30 nm的颗粒，并且在其他方面与真实颗粒显著不同。然而，通过向组装系统中加入肌醇磷酸或磷脂酰肌醇磷酸，在体外获得具有真实病毒体的大小和其他性质的颗粒。因此，HIV-1 Gag蛋白分子之间的相互作用通过肌醇衍生物的结合而改变;这种结合显然是正常HIV-1颗粒组装所必需的。这种要求在基质结构域内缺少残基16-99的缺失Gag蛋白中没有看到。
HIV-1 Gag protein assembles into 100- to 120-nm diameter particles in mammalian cells. Recombinant HIV-1 Gag protein assembles in a fully defined system in vitro into particles that are only 25-30 nm in diameter and that differ significantly in other respects from authentic particles. However, particles with the size and other properties of authentic virions were obtained in vitro by addition of inositol phosphates or phosphatidylinsitol phosphates to the assembly system. Thus, the interactions between HIV-1 Gag protein molecules are altered by binding of inositol derivatives; this binding is apparently essential for normal HIV-1 particle assembly. This requirement is not seen in a deleted Gag protein lacking residues 16-99 within the matrix domain.