Collagen-Induced Arthritis Models
Collagen-Induced Arthritis Models
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DOI：
10.1007/978-1-4939-8802-0_1
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发表时间：
2018-01-01
期刊：
影响因子：
--
通讯作者：
Liu, Shuang
中科院分区：
文献类型：
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作者：
Miyoshi, Maya;Liu, Shuang
由于类风湿性关节炎（RA）研究中使用患者样本进行系统动力学研究的局限性，动物模型有助于进一步了解RA的病理生理学并寻找潜在的治疗靶点或策略。胶原诱导关节炎（CIA）模型是临床前研究中使用的标准 RA 模型之一。 CIA模型与RA有一些共同的病理特征，例如耐受性的破坏和针对胶原蛋白的自身抗体的产生、滑膜炎症细胞浸润、滑膜增生、软骨破坏和骨侵蚀。本章描述了在小鼠中成功诱导 CIA 的方案。在此方案中，通过在易感 DBA/1 小鼠中接种弗氏佐剂中的 II 型异源胶原蛋白进行主动免疫来诱导 CIA。
Due to limitations of using patient-derived samples for systemic kinetic studies in rheumatoid arthritis (RA) research, animal models are helpful for further understanding the pathophysiology of RA and seeking potential therapeutic targets or strategies. The collagen-induced arthritis (CIA) model is one of the standard RA models used in preclinical research. The CIA model shares several pathological features with RA, such as breach of tolerance and generation of autoantibodies targeting collagen, synovial inflammatory cell infiltration, synovial hyperplasia, cartilage destruction, and bone erosion. In this chapter, a protocol for successful induction of CIA in mice is described. In this protocol, CIA is induced by active immunization by inoculation with type II heterologous collagen in Freund's adjuvant in susceptible DBA/1 mice.