Genes expressed during the development and ripening of watermelon fruit
Genes expressed during the development and ripening of watermelon fruit
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DOI：
10.1007/s00299-006-0163-0
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发表时间：
2006-11-01
影响因子：
6.2
通讯作者：
King, S.
中科院分区：
文献类型：
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作者：
Levi, A.;Davis, A.;King, S.
利用西瓜3个不同发育时期的果肉基因，构建了归一化的基因文库，并与叶片基因进行杂交，得到一个差减文库。从5‘端对西瓜果肉消减文库的随机克隆进行了测序，以寻找与坐果、发育和成熟相关的潜在信息基因。将1,046个5‘端序列(表达序列标签；EST)组装成832个非冗余序列，命名为“EST-uniges”。在这832个“EST-uniges”中，有254个(类似于30%)与迄今为止发表的其他植物物种的序列没有显著的同源性。此外，168个“EST-uniges”(约占20%)对应于功能未知的基因，而410个“EST-uniges”(约占50%)对应于其他植物中功能已知的基因。这些EST单基因主要与代谢、膜转运、细胞骨架的合成和结构、细胞壁的形成和细胞分裂、信号转导、核酸结合和转录因子、防御和应激反应以及次生代谢有关。这项研究为科学界提供了西瓜新的遗传信息，并扩大了西瓜果实发育相关基因库。这些基因将成为未来西瓜及其发育的遗传和功能基因组研究的有用靶点。
A normalized cDNA library was constructed using watermelon flesh mRNA from three distinct developmental time-points and was subtracted by hybridization with leaf cDNA. Random cDNA clones of the watermelon flesh subtraction library were sequenced from the 5' end in order to identify potentially informative genes associated with fruit setting, development, and ripening. One-thousand and forty-six 5'-end sequences (expressed sequence tags; ESTs) were assembled into 832 non-redundant sequences, designated as "EST-unigenes". Of these 832 "EST-unigenes", 254 (similar to 30%) have no significant homology to sequences published so far for other plant species. Additionally, 168 "EST-unigenes" (similar to 20%) correspond to genes with unknown function, whereas 410 "EST-unigenes" (similar to 50%) correspond to genes with known function in other plant species. These "EST-unigenes" are mainly associated with metabolism, membrane transport, cytoskeleton synthesis and structure, cell wall formation and cell division, signal transduction, nucleic acid binding and transcription factors, defense and stress response, and secondary metabolism. This study provides the scientific community with novel genetic information for watermelon as well as an expanded pool of genes associated with fruit development in watermelon. These genes will be useful targets in future genetic and functional genomic studies of watermelon and its development.