Labeling efficiency and biodistribution of Technetium-99m labeled nanoparticles: interference by colloidal tin oxide particles
Labeling efficiency and biodistribution of Technetium-99m labeled nanoparticles: interference by colloidal tin oxide particles
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DOI：
10.1016/j.ijpharm.2004.09.022
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发表时间：
2005-01-31
影响因子：
5.8
通讯作者：
Maitra, AN
中科院分区：
文献类型：
--
作者：
Banerjee, T;Singh, AK;Maitra, AN
用硼氢化钠代替常用的亚锡盐作为还原剂，将Tc-99 m（VII）还原到低价态，克服了胶体氧化锡对（99 m）Tc标记壳聚糖纳米粒生物分布的干扰。氯化亚锡法制备的放射性标记壳聚糖纳米粒的生物分布显示放射性主要定位于肝脏和脾脏，而硼氢化钠法制备的放射性标记壳聚糖纳米粒的生物分布显示，即使在2 h后，血液中的放射性也高达10%。有趣的是，在后一种情况下，随着时间的推移放射性降低并不表现为肝脏活性增加。相反，在胃中观察到放射性物质蓄积呈时间依赖性增加。从结果可以得出结论，生物分布的强烈影响的存在下，锡氧化物和Tc-99 m标记的壳聚糖纳米粒子是RES回避和长循环的血液中时，Tc（VII）被还原硼氢化钠，而不是氯化亚锡在放射性标记过程中。(C)2004年由Elsevier B. V.出版
The interference of colloidal tin oxides on the biodistribution of (99m)Technetium radiolabeled chitosan nanoparticles has been overcome by using sodium borohydride instead of commonly used stannous salts as reducing agent for the reduction of Tc-99m (VII) to lower valency states. Biodistribution of radiolabeled chitosan nanoparticles prepared by using stannous chloride method revealed localization of the radioactivity mainly in the liver and spleen while that of radiolabeled chitosan nanoparticles prepared by using sodium borohydride method manifested the presence of radioactivity in blood up to an extent of 10% even after 2 h. Interestingly, the reduction of radioactivity in the latter case with the progress of time was not manifested through an increase in activity in the liver. Rather, a time dependent increased accumulation of radioactive materials was observed in the stomach. From the results it has been concluded that the biodistribution is strongly influenced by the presence of colloidal particles of tin oxides and Tc-99m labeled chitosan nanoparticles are RES evading and long circulating in blood when Tc (VII) is reduced by sodium borohydride and not by stannous chloride during radiolabeling process. (C) 2004 Published by Elsevier B.V.