Genomic Organization of the B3-Domain Transcription Factor Family in Grapevine (Vitis vinifera L.) and Expression during Seed Development in Seedless and Seeded Cultivars
Genomic Organization of the B3-Domain Transcription Factor Family in Grapevine (Vitis vinifera L.) and Expression during Seed Development in Seedless and Seeded Cultivars
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DOI：
10.3390/ijms20184553
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发表时间：
2019-09-02
影响因子：
5.6
通讯作者：
Wang, Xiping
中科院分区：
文献类型：
--
作者：
Ahmad, Bilal;Zhang, Songlin;Wang, Xiping
植物特异性b3域转录因子家族的成员具有重要而多样的功能，特别是在营养和生殖生长方面。虽然B3基因已经在许多其他植物中得到了研究，但关于葡萄B3基因的基因组组织和表达的信息有限。在这项研究中，我们在葡萄基因组中鉴定了50个B3基因，并从染色体定位和合成关系、内含子-外显子组织和启动子顺式元素含量等方面分析了这些基因。我们还从结构域结构和系统发育关系方面分析了假定的蛋白质。根据结果，我们将这些基因分为五个亚家族。研究结果表明，大约一半的基因是由基因组重复引起的，这有助于葡萄B3家族的扩展。顺式元素组成分析表明，这些基因中的大多数可能对激素、光和压力起作用。我们还分析了B3家族成员在葡萄植物不同结构中的表达，包括种子在种子发育过程中的表达。许多B3基因在成熟植物的一个或多个结构中优先表达，表明它们在生长发育中具有特定的作用。此外，在具有代表性的有籽和无籽品种的早期发育种子中，有几个基因的表达存在差异，表明这些基因在种子发育或败育中起作用。本研究结果为葡萄B3基因的功能分析奠定了基础，并为今后葡萄分子育种提供了新的资源。
Members of the plant-specific B3-domain transcription factor family have important and varied functions, especially with respect to vegetative and reproductive growth. Although B3 genes have been studied in many other plants, there is limited information on the genomic organization and expression of B3 genes in grapevine (Vitis vinifera L.). In this study, we identified 50 B3 genes in the grapevine genome and analyzed these genes in terms of chromosomal location and syntenic relationships, intron-exon organization, and promoter cis-element content. We also analyzed the presumed proteins in terms of domain structure and phylogenetic relationships. Based on the results, we classified these genes into five subfamilies. The syntenic relationships suggest that approximately half of the genes resulted from genome duplication, contributing to the expansion of the B3 family in grapevine. The analysis of cis-element composition suggested that most of these genes may function in response to hormones, light, and stress. We also analyzed expression of members of the B3 family in various structures of grapevine plants, including the seed during seed development. Many B3 genes were expressed preferentially in one or more structures of the developed plant, suggesting specific roles in growth and development. Furthermore, several of the genes were expressed differentially in early developing seeds from representative seeded and seedless cultivars, suggesting a role in seed development or abortion. The results of this study provide a foundation for functional analysis of B3 genes and new resources for future molecular breeding of grapevine.