Study on the Relationship Between Microbial Composition and Living Environment in Important Medical Mites Based on Illumina MiSeq Sequencing Technology
Study on the Relationship Between Microbial Composition and Living Environment in Important Medical Mites Based on Illumina MiSeq Sequencing Technology
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基于Illumina MiSeq测序技术研究重要医学螨微生物组成与生存环境的关系
DOI：
10.1093/jme/tjaa034
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发表时间：
2020-07-01
影响因子：
2.1
通讯作者：
Hu, Li
中科院分区：
文献类型：
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作者：
Guo, Yijie;Wang, Ruiling;Hu, Li
螨虫的微生物群与其生长、发育和致病性密切相关。因此，有必要对螨虫体内的细菌进行研究。在此，首次基于16s rRNA V3-V4区域，利用Illumina MiSeq测序技术对医学重要螨类6科9种45个样本的微生物群进行分析。结果显示，在门水平上，变形菌门（56.20-86.40%）占优势，其次是厚壁菌门（6.41-19.43%）、拟杆菌门。 （5.56-13.38%）和放线菌（1.93-28.07%）。但不同属螨的微生物群不同，表现出四个特点：1）微生物群与寄生宿主相关。寄生于人类的毛囊蠕形螨（螨形：蠕形螨科）和短蠕形螨（螨形：蠕形螨科）显示出相似的微生物组成，寄生于狗的犬蠕形螨（螨形：蠕形螨科）和犬疥螨（螨形：蠕形螨科）也表现出相似的微生物组成，但山羊蠕形螨（螨形：蠕形螨科）也表现出相似的微生物组成。寄生羊表现出独特的微生物群落； 2) 微生物群与螨虫的种类相关。 Dermatophagoides farinae 和 Cheyletus malaccensis（螨形目：Cheyletidae）均从面粉中采集，显示出各自的微生物组成； 3）微生物群与生命阶段有关。 D. farinae 成虫和幼虫之间的微生物群存在差异，但在 Psoroptes cuniculi（蜱螨目：Psoroptidae）中没有观察到差异； 4）微生物群与摄血状态有关。吸食血液的巴科鸟虫（寄生形目：Macromonyssidae）成虫的微生物群显着高于未喂食的成虫。这表明螨虫的微生物群受到螨种、寄生宿主、生长阶段和栖息地的影响。因此，了解这些影响因素对于螨传疾病的防治具有非常重要的指导意义。
The microbiota of mites is closely related to their growth, development, and pathogenicity. Therefore, it is necessary to study the bacteria in mites. Here, for the first time, based on 16s rRNA V3-V4 region, the microbiota of 45 samples of nine species in six families of medically important mites were analyzed using Illumina MiSeq sequencing technique.The results showed that, at the phylum level, Proteobacteria (56.20-86.40%) were the dominant, followed by Firmicutes (6.41-19.43%), Bacteroidetes (5.56-13.38%) and Actinobacteria (1.93-28.07%). But at the genera the microbiota of mites are different, showing four characteristics: 1) The microbiota is related to the parasitic host. Demodex folliculorum (Acariforms: Demodicidae) and D. brevis (Acariforms: Demodicidae), both parasitizing humans, showed similar microbial composition, as did D. canis (Acariforms: Demodicidae) and Sarcoptes scabiei canis (Acariforms: Sarcoptidae) parasitizing dogs, but D. caprae (Acariforms: Demodicidae) parasitizing sheep showed unique microbial community; 2) The microbiota is related to mite's species. Dermatophagoides farinae and Cheyletus malaccensis (Acariforms: Cheyletidae), both collecting from flour, show respective microbial composition; 3) The microbiota is related to the life stage. There were differences in microbiota between adults and larvae of D. farinae, but no differences observed in Psoroptes cuniculi (Acariforms: Psoroptidae); and 4) The microbiota is related to the blood-feeding state. The microbiota of blood-fed Ornithonyssus bacoti (Parasitiformes: Macronyssidae) adults was significantly higher than that of unfed adults. This indicates that the microbiota of mites is affected by mite species, parasitic host, growth stage and habitat. Therefore, understanding these influencing factors will have a very important guiding significance for the prevention and control of mite-borne diseases.