High-Throughput Sequencing Reveals Circulating miRNAs as Potential Biomarkers for Measuring Puberty Onset in Chicken (Gallus gallus).
High-Throughput Sequencing Reveals Circulating miRNAs as Potential Biomarkers for Measuring Puberty Onset in Chicken (Gallus gallus).
复制标题
高通量测序表明，循环中的miRNA是测量鸡肉青春期发作的潜在生物标志物（Gallus Gallus）。
DOI：
10.1371/journal.pone.0154958
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Han W;Zhu Y;Su Y;Li G;Qu L;Zhang H;Wang K;Zou J;Liu H
仍然没有高度敏感和独特的生物标志物来测量青春期的开始。循环中的miRNA已被证明是用于诊断各种疾病的有前景的生物标志物。为了鉴定可作为鸡（Gallus gallus）青春期开始测量的生物标志物的循环miRNA，进行Solexa深度测序以分析来自青春期开始前（BO）和青春期开始后（AO）的两个不同青春期阶段的母鸡的血清和血浆中的miRNA表达谱。197个保守的和19个新的miRNAs（reads > 10）被鉴定为鸡血清/血浆中表达的miRNAs。血清和血浆中常见的miRNA量及其从BO到AO的表达变化非常相似，表明不同处理产生血清和血浆对miRNA的影响很小。130个血清miRNAs在BO和AO中差异表达（reads > 10，P < 0.05），68个上调，62个下调。4829个推定基因被预测为40个最差异表达的miRNAs的靶基因，|log 2（倍数变化）|>1.0，P < 0.01）。功能分析揭示了几个途径，与青春期的开始。进一步的实时荧光定量PCR（RT-qPCR）检测发现，miR-29 c、miR-375、miR-215、miR-217、miR-19 b、miR-133 a和let-7a等7种miRNA具有很好的检测鸡青春期发育的潜力。由于miRNAs的高度保守性，这些发现可以为测量其他动物以及人类的青春期开始提供线索。
There are still no highly sensitive and unique biomarkers for measurement of puberty onset. Circulating miRNAs have been shown to be promising biomarkers for diagnosis of various diseases. To identify circulating miRNAs that could be served as biomarkers for measuring chicken (Gallus gallus) puberty onset, the Solexa deep sequencing was performed to analyze the miRNA expression profiles in serum and plasma of hens from two different pubertal stages, before puberty onset (BO) and after puberty onset (AO). 197 conserved and 19 novel miRNAs (reads > 10) were identified as serum/plasma-expressed miRNAs in the chicken. The common miRNA amounts and their expression changes from BO to AO between serum and plasma were very similar, indicating the different treatments to generate serum and plasma had quite small influence on the miRNAs. 130 conserved serum-miRNAs were showed to be differentially expressed (reads > 10, P < 0.05) from BO to AO, with 68 up-regulated and 62 down-regulated. 4829 putative genes were predicted as the targets of the 40 most differentially expressed miRNAs (|log2(fold-change)|>1.0, P < 0.01). Functional analysis revealed several pathways that were associated with puberty onset. Further quantitative real-time PCR (RT-qPCR) test found that a seven-miRNA panel, including miR-29c, miR-375, miR-215, miR-217, miR-19b, miR-133a and let-7a, had great potentials to serve as novel biomarkers for measuring puberty onset in chicken. Due to highly conserved nature of miRNAs, the findings could provide cues for measurement of puberty onset in other animals as well as humans.