Role of allene oxide cyclase in the regulation of rice phenolic acids synthesis and allelopathic inhibition on barnyardgrass
Role of allene oxide cyclase in the regulation of rice phenolic acids synthesis and allelopathic inhibition on barnyardgrass
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DOI：
10.1007/s10725-015-0131-1
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发表时间：
2016-07
影响因子：
4.2
通讯作者：
C. Fang;Yan Yu;Weisi Chen;Xin Jian;Qingshui Wang;Haiping Zheng;Wenxiong Lin
中科院分区：
文献类型：
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作者：
C. Fang;Yan Yu;Weisi Chen;Xin Jian;Qingshui Wang;Haiping Zheng;Wenxiong Lin
茉莉酸(JA)和茉莉酸甲酯是重要的内源信号分子，可以诱导植物对生物和非生物胁迫的抗性。丙二烯氧化环化酶(AOC)是茉莉酸生物合成的关键酶。本研究以化感水稻PI312777和非化感水稻柠檬为材料，分别利用RNA干扰(RNAi)和过表达(OX)技术对AOC基因表达进行了遗传操作，以探讨AOC转录本水平与水稻化感潜力的相关性。与野生型相比，AOC-OX转基因水稻内源JA含量增加，苯丙氨酸解氨酶、肉桂酸-4-羟基酶、羟基酶、辅酶A连接酶、O-甲基转移酶和肉桂醇脱氢酶的转录水平也上调。AOC-OX转基因株系中原儿茶酸、对羟基苯甲酸、阿魏酸、苯甲酸、4-羟基-3-甲氧基苯甲酸和肉桂酸含量较高。AOC-OX转基因株系中酚酸类物质的含量增加，提高了对无芒草的抑制率，而AOC-RNAi转基因株系中的酚酸含量则相反。这些结果表明，AOC可以调节水稻酚酸的合成，从而抑制无芒草的化感作用。
Jasmonic acid (JA) and methyl jasmonate are important endogenous signal molecules that can induce plant resistance to biotic and abiotic stresses. Allene oxide cyclase (AOC) is a key enzyme in the biosynthesis of jasmonates. Here, in order to evaluate the correlation betweenAOCtranscript level and the allelopathic potential of rice, gene expression ofAOCwas genetic operated in allelopathic rice PI312777 and non-allelopathic rice Lemont, using RNA interference (RNAi) and overexpression (OX) techniques respectively. An increase in endogenous JA was found inAOC-OX rice, and the transcript levels of phenylalanine ammonia-lyase, cinnamate-4-hydroxylase, hydroxylase, CoA-ligases,O-methyltransferases, and cinnamoyl alcohol dehydrogenases, which are involved in phenolic acid synthesis, were also up-regulated inAOC-OX transgenic rice when compared with those of the wild type. Higher accumulation of protocatechuic acid,p-hydroxy benzoic acid, ferulic acid, benzoic acid, 4-hydroxy-3-methoxybenzoicacid, and cinnamic acid content were found in theAOC-OX transgenic line. The increase of phenolic acids in theAOC-OX transgenic lines led to enhanced inhibitory rates on barnyardgrass, whereas the reverse was true in theAOC-RNAi transgenic line. The results suggest thatAOCcan regulate the synthesis of rice phenolic acids and thus allelopathic inhibition on barnyardgrass.