CONSTITUTIVE EXPRESSION OF THE ID1 GENE IMPAIRS MOUSE B-CELL DEVELOPMENT
CONSTITUTIVE EXPRESSION OF THE ID1 GENE IMPAIRS MOUSE B-CELL DEVELOPMENT
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DOI：
10.1016/0092-8674(94)90078-7
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发表时间：
1994-12-02
期刊：
影响因子：
64.5
通讯作者：
SUN, XH
中科院分区：
文献类型：
--
作者：
SUN, XH
Id蛋白是碱性螺旋-环-螺旋（bHLH）转录因子的抑制剂。在B细胞谱系中，Id1和Id2基因在前B细胞中表达，并在分化过程中下调。为了确定bHLH蛋白在B细胞发育中的作用以及ld基因下调的意义，我们构建了组成型表达Id1基因的转基因小鼠。他们的表型表明B细胞发育在早期阶段受损。首先，这些小鼠骨髓中B220(+) IgM（+）成熟B细胞或B220(+) CD43（-）前B细胞较少，免疫球蛋白位点V(D)J和V(kappa)J（kappa）重组频率降低，免疫球蛋白、rag1、rag2和lambda 5基因表达水平降低。
The Id proteins are inhibitors of the basic-helix-loop-helix (bHLH) transcription factors. In the B cell lineage, the Id1 and Id2 genes are expressed in pro-B cells and down-regulated during differentiation. To determine the role of bHLH proteins and the significance of downregulation of ld genes in B cell development, transgenic mice constitutively expressing the Id1 gene were generated. Their phenotype suggests that B cell development is impaired at an early stage. Primarily, these mice have few B220(+) IgM(+) mature B or B220(+) CD43(-) pre-B cells in the bone marrow, reduced frequencies of V(D)J and V(kappa)J(kappa) recombination of the immunoglobulin loci, and lower expression levels of the immunoglobulin, RAG-1, RAG-2, and lambda 5 genes.