COMPARISON OF THE BINDING OF RADIOLABELED HUMAN-IGG AND FC FRAGMENTS TO MURINE SPLEEN-CELLS
COMPARISON OF THE BINDING OF RADIOLABELED HUMAN-IGG AND FC FRAGMENTS TO MURINE SPLEEN-CELLS
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DOI：
10.1111/j.1365-3083.1979.tb01368.x
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发表时间：
1979-01-01
影响因子：
3.7
通讯作者：
WEIGLE, WO
中科院分区：
文献类型：
--
作者：
MORGAN, EL;SPIEGELBERG, HL;WEIGLE, WO
在小鼠脾细胞培养物中比较了IG[免疫球蛋白]G1人骨髓瘤蛋白、正常IgG以及各自的Fc和Fab片段的结合特性。125 I标记的IgG和Fc片段都与脾淋巴细胞结合，而Fab片段在最高测试浓度下没有显著结合。在摩尔基础上，比完整IgG结合更多的Fc。根据Scatchard图分析，IgG 1的亲和常数为1.5 × 10 - 6。106 .+-。1倍。105 l/M [分子]，Fc片段的是7.8 × 105 l/M [分子]。105 .+-. 2.6. times. 105 l/M。计算得出IgG 1约有25，000个结合位点/细胞，Fc约有102，000个结合位点/细胞。Fc制备物的解聚没有改变这些值，表明IgG和Fc结合的差异不是由Fc聚集引起的。未标记的IgG抑制约25%的标记的Fc结合，而未标记的Fc抑制约80%的标记的Fc结合。IgG抗原-抗体复合物抑制75%的Fc结合。在倒数实验中，完整IgG和Fc抑制标记IgG的结合达100%。脾细胞制备物中结合IgG和Fc片段的主要细胞群是B [骨髓来源]淋巴细胞。去除巨噬细胞并不显著影响标记的Fc片段的结合。T[胸腺衍生]细胞富集群体结合的Fc片段数量微不足道。
Binding properties of an Ig[immunoglobulin]G1 human myeloma protein, normal IgG, and the Fc and Fab fragments of each were compared in cultures of murine spleen cells. Both 125I-labeled IgG and Fc fragments bound to splenic lymphocytes, whereas Fab fragments did not bind significantly at the highest concentrations tested. On a molar basis, more Fc bound than intact IgG. According to Scatchard plot analysis, the affinity constant of IgG1 was 1.5 .times. 106 .+-. 1 .times. 105 l/M [molecules] and that of the Fc fragments was 7.8 .times. 105 .+-. 2.6 .times. 105 l/M. Approximately 25,000 binding sites/cell were calculated for IgG1 and 102,000 for Fc. Deaggregation of the Fc preparation did not change these values, suggesting that the difference in binding of IgG and Fc did not result from Fc aggregation. Unlabeled IgG inhibited about 25% of the labeled Fc binding, whereas unlabeled Fc inhibited approximately 80% of the labeled Fc binding. IgG antigen-antibody complexes inhibited 75% of the Fc binding. In the reciprocal experiment intact IgG and Fc inhibited binding of labeled IgG by 100%. The major cell population that bound IgG and Fc fragments in the spleen cell preparation was the B [bone marrow-derived] lymphocytes. Removal of macrophages did not significantly affect binding of labeled Fc fragments. T[thymus-derived]-cell-enriched populations bound an insignificant quantity of Fc fragments.