Comparative study of sulfur utilization and speciation transformation of two elemental sulfur species by thermoacidophilic Archaea Acidianus manzaensis YN-25
Comparative study of sulfur utilization and speciation transformation of two elemental sulfur species by thermoacidophilic Archaea Acidianus manzaensis YN-25
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嗜热嗜酸古菌Manzaensis YN-25对硫的利用和两种元素硫形态转化的比较研究
DOI：
10.1016/j.procbio.2013.09.005
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发表时间：
2013-12-01
影响因子：
4.4
通讯作者：
Zhao, Yi-Dong
中科院分区：
文献类型：
--
作者：
Liu, Hong-Chang;Xia, Jin-Lan;Zhao, Yi-Dong
比较研究了嗜热嗜酸古细菌Acidianus manzaensis YN-25对两种元素硫（正交α-S-8和无定形mu-S）的硫利用和形态转化。细胞生长和硫氧化行为结果表明，mu-S上培养的万座A. manzaensis生长速度更快（比α-S-8提前约24h达到稳定期），并产生更多的H+和SO42-。扫描电镜结果表明，mu-S和α-S-8的表面分别被万座冷杉不同程度地生物腐蚀成疏松孔隙和凹坑。傅里叶变换红外光谱分析表明 mu-S 和 alpha-S-8 均被细胞吸附。 X射线衍射和拉曼光谱分析表明，在A. manzaensis细胞生长后，mu-S大部分转化为α-S-8，而α-S-8并未转化为不同的同素异形体。硫K边XANES光谱拟合结果进一步表明，万座黄芪生长后的mu-S由92.1%的α-S-8和7.9%的mu-S组成，而α-S-8的成分没有变化。 (C) 2013 Elsevier Ltd. 保留所有权利。
The sulfur utilization and speciation transformation of two elemental sulfur species (orthorhombic alpha-S-8 and amorphous mu-S) by thermoacidophilic Archaea strain Acidianus manzaensis YN-25 was comparatively studied. The results of cell growth and sulfur oxidation behavior showed that A. manzaensis cultured on mu-S grew faster (about 24h earlier to reach stationary phase than that on alpha-S-8) and produced more H+ and SO42-. Results of scanning electron microscopy indicated that the surfaces of mu-S and alpha-S-8 were differently bio-corroded by A. manzaensis into loose porosities and pits, respectively. Fourier transform-infrared spectroscopy analysis indicated both mu-S and alpha-S-8 were adsorbed by the cells. X-ray diffraction and Raman spectra analysis indicated that mu-S was mostly converted into alpha-S-8 after A. manzaensis cell growth while alpha-S-8 was not transformed into a different allotrope. The fitted result of sulfur K edge XANES spectra further showed that the mu-S after growth of A. manzaensis were composed of 92.1% alpha-S-8 and 7.9% mu-S, while no change in composition for alpha-S-8 was found. (C) 2013 Elsevier Ltd. All rights reserved.