Gamete membrane dynamics during double fertilization in Arabidopsis
Gamete membrane dynamics during double fertilization in Arabidopsis
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DOI：
10.4161/psb.24512
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发表时间：
2013-01-01
影响因子：
2.9
通讯作者：
Mori, Toshiyuki
中科院分区：
文献类型：
--
作者：
Igawa, Tomoko;Mori, Toshiyuki
在被子植物的双受精过程中，一个精子细胞与卵细胞受精产生受精卵，而另一个精子细胞与中央细胞受精产生胚乳。尽管细胞表面结构对雄配子和雌配子的相互作用是至关重要的，但关于双受精过程中配子膜动力学的信息很少。在最近的一项研究中，我们利用拟南芥配子膜标记系的活细胞成像技术分析了双受精过程中的配子膜行为。我们观察到，配子融合后，精子膜信号偶尔会残留在雌配子的边界。此外，还检测到进入受精雌配子的精子膜信号。这些结果表明，随着精子内膜组分进入雌配子，雄配子和雌配子之间发生了质膜融合，随后发生了胞质配子。
In the double fertilization of angiosperms, one sperm cell fertilizes an egg cell to produce a zygote, whereas the other sperm cell fertilizes a central cell to give rise to an endosperm. There is little information on gamete membrane dynamics during double fertilization even though the cell surface structure is critical for male and female gamete interactions. In a recent study, we analyzed gamete membrane behavior during double fertilization by live-cell imaging with Arabidopsis gamete membrane marker lines. We observed that the sperm membrane signals occasionally remained at the boundary of the female gametes after gamete fusion. In addition, sperm membrane signals entering the fertilized female gametes were detected. These findings suggested that plasma membrane fusion between male and female gametes occurred with the sperm internal membrane components entering the female gametes, and this was followed by plasmogamy.