The role of Staphylococcus aureus adhesins in the pathogenesis of ventricular assist device-related infections
The role of Staphylococcus aureus adhesins in the pathogenesis of ventricular assist device-related infections
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DOI：
10.1086/501366
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发表时间：
2006-04-15
影响因子：
6.4
通讯作者：
Lowy, FD
中科院分区：
文献类型：
--
作者：
Arrecubieta, C;Asai, T;Lowy, FD
心室辅助装置（VAD）是治疗终末期充血性心力衰竭的重要形式。然而，VAD的感染，这通常是由金黄色葡萄球菌引起的，对生存构成了重大威胁。使用一种新的VAD膜和异源乳球菌表达系统的体外结合试验，我们鉴定了3S。金黄色葡萄球菌蛋白凝集因子A（ClfA）和纤连蛋白结合蛋白A和B（FnBPA和FnBPB）是参与粘附VAD聚氨酯膜的主要因素。长植入时间大大降低了粘附性，反映了VAD膜拓扑特征的变化，并且主要由葡萄球菌蛋白中的FnBPA结构域介导。我们还比较了S.金黄色葡萄球菌突变株，并显示其他葡萄球菌成分似乎参与粘附VAD膜。最后，我们证明了ClfA，FnBPA和FnBPB介导植入小鼠主动脉内聚氨酯补片的细菌感染。
Ventricular assist devices ( VADs) are an important form of therapy for end-stage congestive heart failure. However, infection of the VAD, which is often caused by Staphylococcus aureus, poses a major threat to survival. Using a novel in vitro binding assay with VAD membranes and a heterologous lactococcal system of expression, we identify 3 S. aureus proteins-clumping factor A (ClfA) and fibronectin binding proteins A and B ( FnBPA and FnBPB) as the main factors involved in adherence to VAD polyurethane membranes. Adherence is greatly diminished by long implantation times, reflecting a change in topological features of the VAD membrane, and is primarily mediated by the FnBPA domains in the staphylococcal proteins. We also compare the adherence of S. aureus mutant strains and show that other staphylococcal components appear to be involved in adherence to VAD membranes. Finally, we demonstrate that ClfA, FnBPA, and FnBPB mediate bacterial infection of implanted murine intra-aortic polyurethane patches.